Advancement has recently been made on the techniques for separating andmaintaining colonies of red imported fire ants, Solenopsis invicta Buren. A new brood rescuemethod significantly improved the efficiency in separating colony from mound soil.Furthermore, a new method was developed to separate brood from the colony using fire antrepellants. Finally, a cost-effective method was developed to coat containers with dilutedFluon(R) (AGC Chemicals America, Inc, Moorestown, NJ, USA), an aqueouspolytetrafluoroethylene, to prevent housed ants from escaping a container. Usually theoriginal Fluon(R) solution is directly applied to the wall of the containers. Reduced concentrations of Fluon(R) were found to be equally effective in preventing ant escape. The use ofdiluted Fluon(R) solutions to coat the containers was recommended because of environmentaland cost-saving benefits. Application of these new techniques can significantly reduce labor,cost and environmental contamination. This review paper collates all the new techniques inone reference which readers can use as a manual.