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目的 探讨甲状腺激素T3对K562细胞铁蛋白表达及其对细胞凋亡和增殖的影响。方法 收集各组细胞胞浆蛋白,用放射免疫法检测铁蛋白含量,并用流式细胞术分析各组细胞凋亡百分率及细胞周期变化。结果 中等浓度及高浓度的T3均能增加K562细胞铁蛋白表达,与空白对照相比有显著性差异（P<0.05）,且随T3浓度增加,铁蛋白的表达亦增高。同一浓度T3与K562细胞共培养,随时间延长,铁蛋白表达增加,与空白对照相比具有显著性差异（P<0.01）。24h培养组细胞凋亡率较空白对照略有下降,差异有统计学意义,72h培养组T3=200nM时凋亡百分率明显下降。T3各处理组中S+G2期细胞比例较空白对照组明显减少（P<001）,且72h各组G2+S细胞百分率较24h明显下降。结论 甲状腺激素T3能诱导K562细胞铁蛋白表达增加,且能干预细胞凋亡和细胞增殖周期。
Objective To investigate the effect of thyroid hormone T3 on the expression of ferritin in K562 cells and its effect on apoptosis and proliferation. Methods The cytoplasmic proteins of each group were collected and the content of ferritin was detected by radioimmunoassay. The percentage of apoptotic cells and cell cycle were analyzed by flow cytometry. Results Both moderate and high concentrations of T3 increased ferritin expression in K562 cells, which was significantly different from that of blank control (P <0.05). The expression of ferritin was also increased with the increase of T3 concentration. The same concentration of T3 and K562 cells were co-cultured with prolonged time, ferritin expression increased, compared with the blank control was significantly different (P <0.01). The apoptotic rate of 24 h culture group was slightly lower than that of the blank control group, the difference was statistically significant. The apoptotic percentage of 72 h culture group decreased significantly when T3 = 200 nM. The proportion of cells in S + G2 phase in T3 group was significantly lower than that in blank control group (P <001), and the percentage of G2 + S cells in 72h group was significantly lower than that in 24h group. Conclusions Thyroid hormone T3 can induce the increase of ferritin expression in K562 cells, and can interfere with cell cycle and apoptosis.