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采用离体鉴定方法对小麦抗白粉病特性进行初步研究 ,结果表明 :最适鉴定方法和培养条件是在小麦叶片展开后 ,将其剪成 3～ 5cm长叶段 ,叶面朝上摆放在加有保绿剂的试管中 ,接种白粉菌后置室内 (光强2 0 0 0 LX,温度 1 8～ 2 2℃ )。光照 6HR/ d,待潜育期结束开始观察记录。苗期离体鉴定结果与田间成株期常规鉴定结果基本一致 :周麦 9号的抗病性最强 ,其次是周麦 1 3、豫麦 54、内乡 1 88、赵 92 -2
The results showed that the optimal identification method and culture conditions were as follows: After the wheat leaves were unfolded, the leaves were cut into 3 ~ 5 cm long leaves and the leaves were placed face up Add green preservative test tube, inoculated with powdery mildew and set the room (light intensity 200L, temperature 18 ~ 22 ℃). Light 6HR / d, to be the end of the incubation period began to observe the record. The result of in vitro seedling identification in vitro was basically the same as that of routine identification in the adult plant stage: Zhoumai 9 had the strongest disease resistance, followed by Zhoumai 1 3, Yumai 54, Neixiang 1 88 and Zhao 92 -2