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目的:建立中耳炎颗粒中连翘苷含量的测定方法。方法:采用高效液相色谱法测定中耳炎颗粒连翘苷的含量。结果:连翘苷含量在0.047 5~1.52μg范围内,线性关系良好(r=0.999 9),平均回收率为99.18%,RSD为0.90%(n=5)。结论:所用方法专属性强、重复性好,可用于中耳炎颗粒的质量控制。
Objective: To establish a method for the determination of forsythin in otitis media granules. Methods: To determine the content of forsythin in otitis media by high performance liquid chromatography. Results: Forsythin had a good linearity (r = 0.999 9) within the range of 0.047 5 ~ 1.52 μg with an average recovery of 99.18% and a RSD of 0.90% (n = 5). Conclusion: The method used is specific and reproducible and can be used for the quality control of otitis media particles.