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目的 探讨黄芩甙、川芎嗪是否通过诱导热休克蛋白 (HSP70 )合成增加来保护脑组织。方法 SD大鼠随机分为①正常对照组 ,②热休克处理组 ,③感染性脑水肿组 ,④黄芩甙组 ,⑤川芎嗪组。其中黄芩甙和川芎嗪又分为小剂量、治疗量及大剂量各 3组。采用Western印迹杂交技术检测各组的HSP70的表达。结果 正常对照组、黄芩甙小剂量组、川芎嗪小剂量组及感染性脑水肿组均有一定量的HSP70合成 ,而黄芩甙、川芎嗪的治疗量及大剂量组以及热休克处理组均见明显的HSP70条带。结论 黄芩甙、川芎嗪能诱导脑组织HSP70合成明显增加 ,两药对感染性脑水肿的治疗机制可能与HSP70合成增加有关。
Objective To investigate whether Huangqi or Ligustrazine protects brain tissue by inducing increased heat shock protein (HSP70) synthesis. Methods SD rats were randomly divided into 1 normal control group, 2 heat shock treated group, 3 infectious brain edema group, 4 Huangqi group, 5 Ligustrazine group. Among them, Astragalus and Ligustrazine are divided into three groups: small dose, therapeutic dose and large dose. Western blot hybridization was used to detect the expression of HSP70 in each group. Results There was a certain amount of HSP70 synthesis in normal control group, small dose group of Huangqi, low dose of Ligustrazine group and infectious brain edema group, but the treatment amount of Huangqi, Ligustrazine and large dose group and heat shock treatment group were obvious. HSP70 banding. Conclusion Astragalus and tetramethylpyrazine can induce the increase of HSP70 synthesis in brain tissue. The therapeutic mechanism of both drugs on infectious brain edema may be related to the increase of HSP70 synthesis.