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目的观察补肾活血方对糖尿病小鼠学习记忆功能及中枢神经递质的影响。方法40只ICR小鼠随机分为正常对照组、模型组、尼膜同组和中药组,每组10只。采用四氧嘧啶尾静脉注射复制糖尿病模型,造模4周后灌胃,中药组给予补肾活血方,尼膜同组给予尼膜同,其余两组给予等量蒸馏水,共8周。观察各组小鼠血糖的变化;Morris水迷宫法测定小鼠学习记忆功能;测定大脑皮质去甲肾上腺素(NE)、多巴胺(DA)、5-羟色胺(5-HT)和5-羟吲哚乙酸(5-HIAA)的含量。结果造模后小鼠血糖升高,与正常对照组比较有统计学差异(P<0.05或P<0.01);尼膜同组和中药组小鼠Morris潜伏期均较模型组明显缩短(P<0.01);中药组小鼠大脑皮质NE含量较模型组升高(P<0.01)。结果补肾活血方能够改善糖尿病小鼠学习记忆功能,其作用机制可能与大脑皮质NE含量变化有关。
Objective To observe the effect of Bushen Huoxue Fang on learning and memory function and central neurotransmitters in diabetic mice. Methods Forty ICR mice were randomly divided into normal control group, model group, nimotop group, and traditional Chinese medicine group, with 10 rats in each group. Alloxan was injected into the tail vein to replicate the diabetic model. Four weeks after the injection, the rats were given intragastric administration. The Chinese medicine group was given Bushen Huoxue Fang and the Nimotopong group was given Nimotop with the same amount of distilled water for 8 weeks. The changes of blood glucose were observed in each group; the learning and memory function of mice was determined by Morris water maze test; norepinephrine (NE), dopamine (DA), 5-hydroxytryptamine (5-HT) and 5-hydroxylamine were measured in cerebral cortex. Acetic acid (5-HIAA) content. Results Compared with the normal control group, the blood glucose level of the mice increased after modeling (P<0.05 or P<0.01). The Morris latency period of the Nimotop group and the traditional Chinese medicine group mice was significantly shorter than that of the model group (P<0.01). The content of NE in the cerebral cortex of the Chinese medicine group was higher than that of the model group (P<0.01). Results Bushen Huoxue Decoction can improve learning and memory function in diabetic mice, and its mechanism may be related to the change of NE content in cerebral cortex.