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目的:探讨纳米Ag-SiO2颗粒对小鼠成纤维细胞(L-929)的体外毒性作用。方法:使用透射电镜(TEM)对纳米颗粒进行表征;CCK-8法观察在24 h、48 h、72 h不同浓度纳米颗粒对L-929细胞活性的影响;倒置显微镜观察染毒24 h后细胞形态的改变,并检测细胞上清液中乳酸脱氢酶(LDH)漏出率。结果:L-929细胞的生长活性随着纳米Ag-SiO2颗粒浓度的升高和染毒时间的延长而下降,低浓度组(6.25,12.5μg/mL)与对照组无明显差异,倒置显微镜下细胞形态正常,生长良好。而高浓度组(50,100μg/mL)与对照组有明显差异,透射电镜下观察细胞有明显的空泡变性,培养液上清中LDH活性明显升高。结论:纳米Ag-SiO2颗粒的体外细胞毒性呈剂量-时间依赖关系,低浓度组具有良好的生物相容性,高浓度组具有一定的细胞毒性,其毒性可能与细胞膜的完整性遭到破坏有关.
Objective: To investigate the in vitro toxicity of nano-Ag-SiO2 particles to mouse fibroblasts (L-929). Methods: The nanoparticles were characterized by transmission electron microscopy (TEM). The effect of nanoparticles with different concentration on the activity of L-929 cells was observed by CCK-8 method at 24 h, 48 h and 72 h. The cells were observed by inverted microscope 24 h after exposure Morphological changes were measured and lactate dehydrogenase (LDH) leakage rate in the cell supernatant was measured. Results: The growth activity of L-929 cells decreased with the increase of the concentration of nano-Ag-SiO2 particles and the prolongation of exposure time. There was no significant difference between low concentration group (6.25,12.5μg / mL) and control group. Under inverted microscope Cell morphology is normal, good growth. The high concentration group (50,100μg / mL) and the control group were significantly different, under the transmission electron microscopy showed significant vacuolar degeneration, the supernatant of LDH activity was significantly increased. CONCLUSION: The in vitro cytotoxicity of nano-Ag-SiO2 particles is dose-time dependent. The low concentration group has good biocompatibility. The high concentration group has certain cytotoxicity, and its toxicity may be related to the destruction of cell membrane integrity .