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目的：研究济南假单胞菌的发酵工艺、提取路线及代谢产物的制备，观察其抗肿瘤活性。方法：通过发酵、提取、分离、结晶、重结晶， 得到棕褐色针状结晶（ Ｐｓ１６１） 。结果： Ｐｓ１６１ ０２５ｍｇ／ｋｇ 和０５５ｍｇ／ｋｇ 对 Ｈ２２ 、 Ｕ１４ 均有明显的抑制作用，对 Ｈ２２ 抑瘤率分别为５４７ ％ 和４４１ ％ ， Ｐ＜ ００１ ； Ｕ１４ 为６５２ ％ 和４３９ ％ ， Ｐ＜ ００１ 。采用 Ｍ Ｔ Ｔ 法观察了 Ｐｓ１６１ 对４ 种人类肿瘤细胞株增殖的抑制作用，当 Ｐｓ１６１ ０２２５μｇ／ｍｌ 时，对 Ｈ Ｌ６０ 和小细胞肺癌的抑制作用为１００ ％ ，当浓度减少到０１０μｇ／ｍｌ 时，抑制作用消失；在０６０μｇ／ｍｌ 时，对 Ｋ５６２ 、 Ｓ Ｇ Ｓ７９０１ 的抑制作用为１００ ％ ，当浓度减少到００５μｇ／ｍｌ 以下时，抑制作用消失。结论：药效结果表明， Ｐｓ１６１ 具有明显的抗肿瘤活性，毒性较低，对光、热稳定性良好，是一种具有较好开发前景的抗肿瘤抗生素。
Objective: To study the fermentation process, extraction route and preparation of metabolites of Pseudomonas syringae, and observe its antitumor activity. Method: Through fermentation, extraction, separation, crystallization, recrystallization, tan needle-like crystals (Ps-161) were obtained. Results: Ps 161 025mg/kg and 055mg/kg had obvious inhibitory effects on H22 and U14, and the inhibitory rate of H22 was 54.7% and 44.1%, respectively. P<0.01. U14 is 652% and 439 %, P< 001. The inhibitory effect of Ps 161 on the proliferation of 4 human tumor cell lines was observed by M T T method. When Ps 161 0 225 μg/ml, the inhibitory effect on H L 60 and small cell lung cancer was 100%. When the concentration is reduced to 010μg/ml, the inhibitory effect disappears; at 060μg/ml, the inhibitory effect on K562 and SGS-7901 is 100%, and when the concentration is reduced below 005μg/ml, the inhibition is suppressed. The effect disappears. Conclusion: The results of efficacy show that Ps161 has obvious anti-tumor activity, low toxicity, good stability to light and heat, and is an anti-tumor antibiotic with good development prospects.