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目的分离筛选出抗肿瘤复方制剂仙慈丹(XCD)的抗肿瘤活性部位。方法采用大孔树脂柱色谱法对抗肿瘤复方制剂XCD水提取液进行分段富集,运用噻唑蓝(MTT)比色法对分段富集得到的部位进行体外抗肿瘤活性测定。结果抗肿瘤复方制剂XCD水提取液过AB-8型树脂纯化得到的P2(体积分数20%乙醇洗脱液)、P3(体积分数40%乙醇洗脱液)、P4(体积分数60%乙醇洗脱液)、P5(体积分数80%乙醇洗脱液)对人肝癌HepG2细胞表现出较强的抑制作用。结论 XCD水提液过大孔树脂后乙醇洗脱部位是XCD制剂的抗肿瘤活性部位。
Objective To isolate and screen antitumor active sites of anti-tumor compound Xidan Dan (XCD). Methods The aqueous extract of XCD was prepared by macroporous resin column chromatography. The antitumor activity was determined by MTT colorimetric assay. Results The anti-tumor compound XCD water extract was purified by AB-8 resin (volume fraction 20% ethanol eluent), P3 (volume fraction 40% ethanol eluent), P4 Dehydration), P5 (volume fraction of 80% ethanol eluent) on human hepatoma HepG2 cells showed a strong inhibitory effect. Conclusion The eluted fraction of XCD after the macroporous resin is the antitumor active site of XCD preparation.