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观察不同参数(频率、脉宽、峰值)组合的陡脉冲电场对恶性肿瘤细胞的杀伤效应。将癌细胞悬液3 ml盛于玻璃瓶中,每次施加不同参数组合的电脉冲20 min,用MTT法测细胞死亡率,并制作生长曲线;观察细胞的超微结构变化。结果发现:不同参数组合的陡脉冲对癌细胞的杀伤有较大影响,随着参数值的增大,细胞死亡率上升,细胞的增殖受到抑制;实验组的细胞肿胀,溶解性坏死,仅留细胞轮廓,细胞膜完整性破坏,线粒体空化、肿胀。
To observe the killing effect of steep pulsed electric fields with different parameters (frequency, pulse width and peak value) on malignant tumor cells. 3 ml of the cancer cell suspension was placed in a glass bottle and each time a pulse of different parameters was applied for 20 min. The cell death rate was measured by MTT assay and the growth curve was made. The ultrastructure of the cells was observed. The results showed that the steep pulses with different parameters had a significant impact on the killing of cancer cells. With the increase of the parameter values, the cell death rate increased and the cell proliferation was inhibited. In the experimental group, cell swelling and lytic necrosis were only retained Cell outline, cell membrane integrity destruction, mitochondria cavitation, swelling.