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背景:表皮生长因子可以抑制胃酸分泌,促进溃疡愈合,对胃肠道黏膜具有保护作用,还具有促进黏膜细胞生长和增殖的作用。目的:观察不同剂量表皮生长因子对放射性肠道损伤后大鼠肠黏膜屏障影响的量效观察。设计:随机对照实验。单位:新疆医科大学第一附属医院及公共卫生学院。材料:实验于2001-08/2003-05在新疆医科大学公共卫生学院和新疆医科大学第一附属医院完成。选用新生Wistar大鼠80只,雌雄各半。方法:将Wistar大鼠随机分成阴性,阳性对照组及表皮生长因子30μg/kg,20μg/kg,10μg/kg组,每组16只,雌雄各半。除阴性对照组外,各组大鼠腹部γ射线单次9.5Gy辐射制备动物模型后,皮下注射表皮生长因子1次/d进行干预,8d后观察动物体质量、食物摄入量、食物利用率的变化,并行肠黏膜形态学检查。主要观察指标:①不同剂量表皮生长因子对放射性损伤后大鼠体质量增长、食物摄入量、每日食物摄入量、每日食物利用率的影响。②光镜和电镜下肠黏膜检查结果。结果:①应用表皮生长因子30,20,10μg/kg组大鼠体质量增长与阴性对照组及阳性对照组比较,差异无明显意义(P>0.05)。②应用表皮生长因子30,20,10μg/kg组雄鼠和雌鼠每日食物摄入量与阴性对照组比较,差异有显著意义(P<0.05),与阳性对照组比较差异无显著意义(P>0.05)。③光镜和电镜结果显示,应用表皮生长因子使放射性肠道损伤后肠黏膜形态结构随着表皮生长因子剂量的增加,逐渐改善,其中表皮生长因子30μg/kg组有明显改善,可见上皮细胞显著增生,杯状细胞数目增多。结论:不同剂量表皮生长因子对大鼠体质量、食物摄入量、食物利用率可能没有影响,但大剂量组表皮生长因子对放射性肠道损伤后肠黏膜形态结构的改善,以及肠上皮细胞增生和修复产生促进作用。
Background: Epidermal growth factor can inhibit gastric acid secretion, promote ulcer healing, have protective effect on gastrointestinal mucosa, and also promote mucosal cell growth and proliferation. Objective: To observe the effect of different doses of epidermal growth factor on the intestinal mucosal barrier in rats with radiation-induced intestinal injury. Design: Randomized controlled experiment. Unit: First Affiliated Hospital of Xinjiang Medical University and School of Public Health. MATERIALS: The experiment was performed at the School of Public Health, Xinjiang Medical University and the First Affiliated Hospital of Xinjiang Medical University from August 2001 to May 2003. Eighty newborn Wistar rats were chosen, male and female in half. Methods: Wistar rats were randomly divided into negative group, positive control group and epidermal growth factor 30μg / kg, 20μg / kg, 10μg / kg group, 16 in each group, male and female. Except for the negative control group, rats were injected γ-rays once a single dose of 9.5Gy into the abdominal cavity of rats to induce epidermal growth factor 1 / d subcutaneously. After 8 days, the body weight, food intake, food utilization rate Changes in parallel intestinal mucosal morphology. MAIN OUTCOME MEASURES: ①Effects of different doses of epidermal growth factor on body weight gain, food intake, daily food intake and daily food utilization rate after radiation injury. ② light microscopy and electron microscopy results of intestinal mucosa. Results: ① There was no significant difference in the growth of body mass between the 30, 20, 10μg / kg epidermal growth factor group and the negative control group and positive control group (P> 0.05). ② The daily food intake of male and female mice with epidermal growth factor 30, 20 and 10 μg / kg was significantly different from that of the negative control group (P <0.05), but no significant difference compared with the positive control group P> 0.05). ③ The results of light microscope and electron microscope showed that the morphological changes of intestinal mucosa after radiation-induced intestinal injury increased gradually with the increase of epidermal growth factor dose, and the number of epidermal cells was significantly higher in epidermal growth factor 30μg / kg group Hyperplasia, goblet cells increased. Conclusion: The different doses of epidermal growth factor may have no effect on body weight, food intake and food utilization rate in rats, but the high dose group of epidermal growth factor on intestinal mucosa after radiation-induced intestinal injury morphological improvement, as well as intestinal epithelial cell proliferation And repair produce a boost.