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目的探讨PI3K p85α对人大肠癌细胞侵袭及转移的影响。方法将人大肠癌细胞株LoVo分为3组:空白对照组不给予任何干预措施,阴性转染组转染不含干扰载体的单纯慢病毒,干扰转染组转染包装有慢病毒干扰载体pLL-PI3K p85α-shRNA的质粒,对各组细胞进行伤口愈合实验、迁移实验及侵袭实验。结果转染pLL-PI3K p85α-shRNA之后,干扰转染组细胞PI3K p85α蛋白表达水平较阴性转染组明显下调;伤口愈合实验结果显示,干扰转染组大肠癌细胞愈合能力较空白对照组和阴性转染组下降;迁移实验、侵袭实验结果显示,干扰转染组大肠癌细胞迁移及侵袭能力较空白对照组和阴性转染组下降。结论 PI3K p85αsiRNA转染大肠癌细胞株LoVo,可抑制其侵袭及转移能力,PI3K p85α可能成为大肠癌基因治疗的靶点。
Objective To investigate the effect of PI3K p85α on the invasion and metastasis of human colorectal cancer cells. Methods Human colorectal cancer cell line LoVo was divided into three groups: the blank control group was not given any intervention, the negative transfected group transfected with lentivirus without interfering vector transfection group transfected with lentiviral vector pLL -PI3K p85α-shRNA plasmids, the wound healing assay, migration assay and invasion assay of each group of cells were performed. Results After transfection with pLL-PI3K p85α-shRNA, the expression of PI3K p85α protein in the transfected cells was significantly down-regulated compared with that in the negative transfected cells. The results of wound healing assay showed that the interference ability of the transfected cells was higher than that of the blank control group and negative Transfection group decreased; migration experiments, invasion experiments showed that transfection group colorectal cancer cell migration and invasion than the blank control group and negative transfected group decreased. Conclusion PI3K p85α siRNA transfection can inhibit the invasion and metastasis of colorectal cancer cell line LoVo, and PI3K p85α may be the target of gene therapy for colorectal cancer.