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目的:建立实用、有效的皮肤光老化Sprague-Dawley大鼠模型,并探讨该疾病模型的评价标准。方法:SD大鼠随机分为实验组和对照组,每组8只。采用长波紫外线结合中波紫外线模拟日光照射实验组大鼠背部皮肤,每天一次,照射总剂量递增,持续10周。光照结束后,观察皮肤外观、组织病理、超微结构及生化改变。结果:10周结束后,实验组大鼠较对照组皮肤粗糙、缺乏弹性、出现明显深大皱纹。表皮增厚、腺体明显增生、胶原纤维减少,超微结构下可见老化坏死细胞增多;对皮肤组织进行检测,实验组丙二醛含量升高[(10.43±5.67)nmol/mgprot,P=0.009]、羟脯氨酸减少[(7.37±1.15)μg/mg,P=0.010]较对照组有统计学差异,而超氧化物歧化酶的活性[(21.09±8.60)U/mgprot,P=0.401]改变无统计学差异。结论:实验组SD大鼠皮肤具备光老化的典型特征,表明利用紫外线剂量递增、维持10周的方法可成功构建皮肤光老化动物模型,并为评价皮肤光老化程度提供了全面系统的技术参数。
Objective: To establish a practical and effective skin photoaging Aging Sprague-Dawley rat model and to evaluate the evaluation criteria of the disease model. Methods: SD rats were randomly divided into experimental group and control group, with 8 rats in each group. The long-wave UV and UV-B radiation were used to simulate the skin of the dorsal skin of the experimental group, once a day. The total dose of irradiation increased gradually for 10 weeks. After lighting, observe the skin appearance, histopathology, ultrastructure and biochemical changes. Results: At the end of 10 weeks, the skin of the experimental group was rougher than the control group, with a lack of elasticity and obvious deep wrinkles. Epidermis thickening, gland hyperplasia, collagen fibers decreased, and ultrastructural changes of necrotic cells were observed. The skin tissues were detected in the experimental group with increased MDA level (10.43 ± 5.67 nmol / mgprot, P = 0.009 ], Hydroxyproline decreased [(7.37 ± 1.15) μg / mg, P = 0.010] compared with the control group, while the activity of superoxide dismutase [(21.09 ± 8.60) U / mgprot, P = 0.401 No significant difference in change. Conclusion: The skin of experimental rats possesses typical characteristics of photo-aging. It indicates that the skin photo-aging animal model can be successfully constructed by increasing the dose of UV light for 10 weeks and provides a comprehensive and systematic technical parameter for evaluating the photoaging of skin.