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目的:应用基于超高压液相色谱-质谱联用(UPLC-MS)的代谢组学平台研究有氧运动对高脂饲料诱导高脂血症的治疗效果,阐明其生物化学作用机制。方法:用高脂饲料喂饲大鼠6周,同时进行有氧运动干预。采用UPLC-MS测定正常组、模型组及有氧运动组大鼠血清,采用偏最小二乘判别分析法研究对照组、模型组及有氧运动组之间的代谢物谱差异,采用Markerlynx软件鉴定生物标示物。结果:结果显示鉴定的代谢产物包括9个溶血卵磷脂、5个脂肪酸、2个鞘脂和1个氨基酸;在高脂血症模型中,高脂饲料干扰了溶血卵磷脂和脂肪酸类代谢产物的正常代谢;溶血卵磷脂和脂肪酸类代谢产物是是高脂饲料诱导的高脂血症的生物标示物;有氧运动对鉴定的生物标示物存在不同程度的逆转作用,改善了异常的脂质代谢途径。结论:研究表明,UPLC-MS平台可用于观察有氧运动对高脂血症治疗的大鼠血清代谢组学变化,溶血卵磷脂和脂肪酸类代谢产物是高脂饲料诱导的高脂血症的生物标示物,溶血卵磷脂和脂肪酸的异常代谢是高脂血症的主要生物化学作用机制。有氧运动对这些生物标示物有不同程度的逆转作用,表明有氧运动对高脂血症有一定程度的治疗作用。
OBJECTIVE: To study the therapeutic effect of aerobic exercise on hyperlipidemia induced by high-fat diet by using UPLC-MS based metabonomics platform and to elucidate its biochemical mechanism. Methods: The rats were fed with high-fat diet for 6 weeks, meanwhile aerobic exercise intervention was carried out. UPLC-MS was used to determine the serum of normal rats, model rats and aerobic exercise rats. Partial least squares discriminant analysis was used to study the difference of metabolites between control group, model group and aerobic exercise group. Markerlynx software was used to identify the differences Biomarkers. Results: The results showed that the identified metabolites include 9 lysophosphatidylcholine, 5 fatty acids, 2 sphingolipids and 1 amino acid. In the model of hyperlipidemia, high-fat diet interfered with lysophosphatidylcholine and fatty acid metabolites Normal metabolism; lysophosphatidylcholine and fatty acid metabolites are hyperlipidemia-induced hyperlipidemia biomarkers; aerobic exercise on the identification of biomarkers have varying degrees of reversal, improved abnormal lipid metabolism way. CONCLUSIONS: The results show that the UPLC-MS platform can be used to observe the changes of serum metabolomics in hyperlipidemic rats treated with aerobic exercise. The lysolecithin and fatty acid metabolites are hyperlipidemia-induced creatures Abnormal metabolism of label, lysophosphatidylcholine and fatty acids is the major biochemical mechanism of hyperlipidemia. Aerobic exercise on these biomarkers have varying degrees of reversal, indicating that aerobic exercise on hyperlipidemia have a degree of therapeutic effect.