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目的 :观察2种荧光原位杂交(fluorescence in situ hybridization,FISH)探针检测Myc基因在弥漫大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)中的异常形式及意义。方法:选用Myc断裂分离探针以及IgH/Myc/8号染色体着丝粒探针(chromosome enumeration probe 8,CEP8)双融合探针对40例DLBCL患者的淋巴结进行FISH检测,并选择20例反应性增生淋巴结作为对照用于FISH探针正常阈值的确立。应用免疫组织化学法检测40例DLBCL患者淋巴结中Myc蛋白的表达。结果 :Myc断裂分离探针检测有3种荧光信号模式,40例DLBCL患者中有5例(12.5%)患者发生Myc基因重排,7例(17.5%)患者发生Myc基因扩增,28例(70.0%)患者为正常荧光模式。IgH/Myc/CEP8双融合探针检测可出现5种荧光信号模式,40例DLBCL患者中有3例(7.5%)患者发生IgH/Myc基因融合,5例(12.5%)患者发生Myc基因的低拷贝数获得,3例(7.5%)患者出现Myc基因扩增,6例(15.0%)患者表现为8号染色体多体,23例(57.5%)患者为正常荧光模式。40例DLBCL患者中,Myc蛋白阳性者10例,其中5例出现Myc基因重排或IgH/Myc基因融合,3例出现Myc基因扩增。结论 :2种不同的Myc荧光探针用于DLBCL检测会出现多种异常信号模式,二者相互结合可以准确发现Myc基因的异常。
OBJECTIVE: To observe the abnormality and significance of Myc gene in diffuse large B-cell lymphoma (DLBCL) by using two fluorescence in situ hybridization (FISH) probes. Methods: The lymph nodes of 40 patients with DLBCL were examined by Myc cleavage and IgE / Myc / chromosome enumeration probe 8 (CEP8) double fusion probe, and 20 patients with reactivity Hyperplastic lymph nodes served as controls for the establishment of normal thresholds for FISH probes. Immunohistochemistry was used to detect the expression of Myc in lymph nodes of 40 patients with DLBCL. Myc gene rearrangement was detected in 5 (12.5%) of 40 DLBCL patients and Myc in 7 (17.5%) patients, and 28 70.0%) patients were in normal fluorescence mode. Five patterns of fluorescence signals can be detected in the IgH / Myc / CEP8 double fusion probe. IgH / Myc fusion occurs in 3 of 40 (7.5%) of 40 DLBCL patients and in 5 (12.5%) of patients The copy number was obtained. Three patients (7.5%) had Myc gene amplification. Six patients (15.0%) showed multiple chromosome 8 and 23 (57.5%) had normal fluorescence pattern. In 40 cases of DLBCL patients, Myc protein positive in 10 cases, of which 5 cases of Myc gene rearrangement or IgH / Myc gene fusion, 3 cases of Myc gene amplification. CONCLUSION: Two different Myc fluorescent probes are used to detect multiple abnormal signal patterns in DLBCL detection. The combination of the two can accurately detect the abnormality of Myc gene.