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研究表明 ,刺芹侧耳液体培养的适宜碳源为葡萄糖和玉米粉 ,适宜氮源为酵母膏和豆饼粉 ,经 0～ 4 8h的适应期 ,4 8～ 12 0h的增殖生长期后进入稳定生长期 ,生物量可达 1 70 g/ 10 0mL (干重 )以上。菌丝体多糖含量为 18 2 9mg/ g ,多糖主要由甘露糖、葡萄糖和半乳糖构成 ,菌丝体氨基酸含量≥ 2 0 0mg/g。多糖的抗氧化活性结果表明 ,在Fe2 + 催化亚油酸脂质过氧化体系及Fenton催化亚油酸脂质过氧化体系中 ,0 5 %的添加量抑制率可高达 4 8 2 0 %和 4 6 72 % ,具有一定的抗氧化效果。
The results showed that the suitable carbon sources for Pleurotus citrinopileatus liquid culture were glucose and corn flour, suitable nitrogen source was yeast extract and bean cake flour, and then entered the stable growth after the 48 ~ 120h proliferative growth period The biomass can reach above 1 70 g / 10 0 mL (dry weight). Mycelium polysaccharide content of 18 2 9mg / g, polysaccharides mainly composed of mannose, glucose and galactose, mycelium amino acid content ≥ 200mg / g. The results of antioxidant activity of polysaccharides showed that in the system of Fe2 + -activated linoleic acid lipid peroxidation and Fenton-catalyzed linoleic acid lipid peroxidation system, the inhibition rate of 0 5% can be up to 4820% and 4 6 72%, with a certain antioxidant effect.