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目的:探讨可溶性内皮素在妊娠期高血压疾病(HDCP)患者血清和胎盘组织中的表达及其与HDCP发病的关系。方法:选取2009年7月～2011年1月在武汉市第一医院妇产科住院分娩的HDCP患者74例,其中包括妊娠期高血压25例、轻度子痫前期26例、重度子痫前期23例;选取同期正常晚期妊娠妇女25例为对照组。采用酶联免疫吸附试验(ELISA)检测各组血清中的内皮素水平;采用免疫组织化学法检测所有患者胎盘组织中的内皮素表达水平。结果:与对照组比较,HDCP组血清及胎盘组织中的内皮素表达水平明显升高(P<0.01)。HDCP各组血清及胎盘组织中的内皮素表达水平比较差异有统计学意义(P<0.05)。血清内皮素及胎盘组织内皮素表达水平呈正相关。结论:孕妇血清及胎盘组织中内皮素表达水平的变化与HD-CP的发病及病情发展有关。
Objective: To investigate the expression of soluble endothelin in serum and placenta of patients with HDCP and its relationship with HDCP. Methods: From July 2009 to January 2011 in Wuhan First Hospital Obstetrics and Gynecology hospital delivery of HDCP in 74 patients, including 25 cases of gestational hypertension, mild preeclampsia in 26 cases, severe preeclampsia Twenty-three cases were selected as normal control group. Twenty-five normal pregnant women in the same period were selected as control group. The level of endothelin in serum of each group was detected by enzyme-linked immunosorbent assay (ELISA). The level of endothelin in placental tissue of all the patients was detected by immunohistochemistry. Results: Compared with control group, the level of endothelin in serum and placenta of HDCP group was significantly increased (P <0.01). The levels of endothelin in serum and placenta of HDCP group were significantly different (P <0.05). Serum endothelin and placental tissue endothelin expression was positively correlated. Conclusion: The change of endothelin expression in serum and placenta of pregnant women is related to the pathogenesis and development of HD-CP.