一株地衣芽孢杆菌碱性蛋白酶的研究Ⅱ.

来源 :微生物学通报 | 被引量 : 0次 | 上传用户：marine_ogz

【摘要】

：

曾分离到一株产碱性蛋白酶的地衣芽孢杆菌011,命名为地衣芽孢杆菌砀山亚种.酶的最适作用条件:pH9.0,60℃.对该菌株进行连续4次不同的物理、化学方法的诱变处理.诱变剂为紫外

【作者】

：

方海红黄红英张林普夏颖罗震平

【机构】

：

安徽师范大学生命科学学院,安徽医科大学微生物教研室,江苏省农业科学院

【出处】

：

微生物学通报

【发表日期】

：

2004年期

【关键词】

：

地衣芽孢杆菌砀山亚种碱性蛋白酶诱变育种

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曾分离到一株产碱性蛋白酶的地衣芽孢杆菌011,命名为地衣芽孢杆菌砀山亚种.酶的最适作用条件:pH9.0,60℃.对该菌株进行连续4次不同的物理、化学方法的诱变处理.诱变剂为紫外线、亚硝酸、和低能N+离子,其处理方式包括单独或复合处理两种.最后获得一株高产碱性蛋白酶的变异株(C3-03)菌株产酶活力从725u/mL提高到12425u/mL.该突变株的最适产酶条件为起始pH8.0～9.0,培养温度32℃～37℃,振荡培养时间44～48h.

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一株地衣芽孢杆菌碱性蛋白酶的研究Ⅱ.

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