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[目的]通过测定慢性苯中毒患者、苯接触者及对照组体检者外周血中调节性T细胞(Treg)数量的变化,探讨慢性苯中毒发生的免疫学机制。[方法]采用流式细胞仪技术对30例慢性苯中毒患者、30例苯接触者及30例对照组体检者外周血标本中单个核细胞的CD4+CD25+Treg细胞进行检测,比较慢性苯中毒患者、苯接触者及对照组体检者之间CD4+CD25+Treg细胞表达的差异。[结果]与对照组比较,慢性苯中毒患者外周血单个核细胞中CD4+CD25+Treg细胞百分比明显降低(P<0.05);与苯接触者比较,慢性苯中毒患者单个核细胞中CD4+CD25+Treg细胞百分比明显降低(P<0.05);苯接触者外周血单个核细胞中CD4+CD25+Treg细胞百分比与对照组相比,差异无统计学意义(P>0.05)。[结论]慢性苯中毒患者存在细胞免疫紊乱。CD4+CD25+Treg细胞百分比的减少可能为慢性苯中毒发生的致病机制之一。CD4+CD25+Treg有可能被作为监测长期苯作业人群的一个生物学指标。
[Objective] To investigate the immunological mechanism of chronic benzene poisoning by measuring the number of Tregs in peripheral blood of patients with chronic benzene poisoning, benzene exposure and control group. [Methods] The levels of CD4 + CD25 + Treg cells in peripheral blood mononuclear cells of 30 chronic benzene poisoning patients, 30 benzene contact patients and 30 control subjects were detected by flow cytometry, and chronic benzene poisoning Differences in the expression of CD4 + CD25 + Treg cells between patients, benzene-exposed subjects and control subjects. [Results] Compared with the control group, the percentage of CD4 + CD25 + Treg cells in peripheral blood mononuclear cells was significantly decreased in patients with chronic benzene poisoning (P <0.05). Compared with benzene exposure, CD4 + CD25 (P <0.05). The percentage of CD4 + CD25 + Treg cells in peripheral blood mononuclear cells from benzene-exposed patients was not significantly different from that of the control group (P> 0.05). [Conclusion] There are cellular immune disorders in patients with chronic benzene poisoning. Decreased percentage of CD4 + CD25 + Treg cells may be one of the pathogenesis of chronic benzene poisoning. CD4 + CD25 + Treg may be used as a biological indicator to monitor long-term benzene population.