论文部分内容阅读
观察脂肪栓塞综合征动物模型表明：①犬的静脉血凝块冰冻切片油红Ｏ染色，可以找到桔红色的脂滴，并可计算出脂滴数量和大小；②取实验犬的肺组织冰冻切片油红Ｏ染色，也可看到大量的桔红色脂滴沉着于肺组织内；③用气相色谱法检测血浆游离脂肪酸时，发现静脉注射骨髓脂肪后游离脂肪酸明显升高；④犬的动脉血氧分压下降，当降至７．９９±０．３０ｋＰａ时，犬出现呼吸困难等症状。上述结果说明，用骨髓脂肪静脉注射建立脂肪栓塞综合征的动物模型是可靠的。
Observe the animal model of fat embolism syndrome showed that: ① canine venous blood clot frozen section oil red O staining, you can find orange lipid droplets, and can calculate the number and size of lipid droplets; ② canine experimental lung tissue frozen sections Oil red O staining, also can see a large number of orange lipid droplets in the lung tissue deposition; ③ gas chromatography for the determination of plasma free fatty acids, found that after intravenous injection of bone marrow fat free fatty acid was significantly increased; ④ canine arterial oxygen Partial pressure drop, when dropped to 7.99 ± 0.30kPa, the dog appeared dyspnea and other symptoms. The above results indicate that the establishment of an animal model of fatty embolism syndrome by intravenous injection of bone marrow fat is reliable.