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目的:通过测定育龄期不孕妇女卵泡早期、采卵日、黄体中期血清白血病抑制因子(LIF)的含量,阐明LIF在血清中的变化规律,探讨血清中LIF与妊娠结局的关系,揭示LIF在胚胎种植过程中的可能作用机制,进而为提高人类辅助生殖技术的胚胎种植率和临床妊娠率提供理论依据。方法:选择在宁夏医科大学附属医院生殖医学中心首次实施体外受精-胚胎移植(IVF-ET)/卵泡浆内单精子显微注射(ICSI)治疗的不孕症妇女48例,采集同一患者自然周期卵泡早期、黄体中期血清以及IVF-ET周期中采卵日的血清及卵泡液,应用ELISA法测定血清及卵泡液中LIF的含量。结果:卵泡早期、采卵日、黄体中期血清LIF含量比较差异有统计学意义(P<0.05),采卵日血清LIF含量明显高于卵泡早期和黄体中期,黄体中期血清LIF含量略高于卵泡早期;妊娠组采卵日血清LIF含量比卵泡早期血清LIF含量的增加值明显高于非妊娠组(P<0.05)。结论:卵泡早期、采卵日、黄体中期血清LIF含量比较差异有统计学意义,提示月经周期中血清LIF来源的部位随时相而异;IVF-ET周期中采卵日血清LIF主要来源于卵巢;卵泡早期血清LIF及采卵日血清LIF的增加幅度可能预测妊娠结局。
OBJECTIVE: To determine the changes of serum LIF in early stage of ovum follicle, ovum day and middle stage of luteal phase in infertile women of childbearing age, to elucidate the relationship between LIF in serum and pregnancy outcome, to reveal the relationship between LIF Which may provide a theoretical basis for improving embryo implantation rate and clinical pregnancy rate of human assisted reproductive technology. Methods: Forty-eight infertile women who underwent in vitro fertilization-embryo transfer (IVF-ET) / intracytoplasmic sperm injection (ICSI) in the Reproductive Medicine Center of Affiliated Hospital of Ningxia Medical University were enrolled in this study. Serum and follicular fluid in early follicle, mid luteal phase and IVF-ET ovulation day were measured by ELISA. The levels of LIF in serum and follicular fluid were measured by ELISA. Results: There was significant difference in serum LIF content between early follicle, ovum day and mid luteal phase (P <0.05), serum LIF content of ovum day was significantly higher than that of early follicle and mid luteal phase, In the early stage, the serum LIF content of the ovulation day of pregnancy group was significantly higher than that of the non-pregnancy group (P <0.05). CONCLUSION: The serum LIF levels in early follicle, ovum harvesting and mid luteal phase have statistical significance, suggesting that the location of serum LIF in the menstrual cycle may vary with time. The serum LIF in the IVF-ET cycle mainly comes from the ovary. Early serum follicle serum LIF and ovum collection serum LIF increase may predict pregnancy outcome.