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1997年,日本学者Nishzama和Okamoto等报告用差异显示分析技术(RDA)首次从1例输血后肝炎患者血清中分离别一种新的DNA病毒,不久又报告,该病毒DNA为单链、线性、无包膜、长约3739bp的细小病毒科(Parvoviridaefamily)成员,并命名为TT病毒。自此,TT病毒受到广泛关注。随着有关该病毒分子生物学和流行病学特征以及致病性等问题的研究不断深入,各种争论以及不同的研究结果也随之产生。最近,有人用很有说服力的研究资料说明TT病毒的基因组结构呈环状而非线性;基因组全长为3852bp,比Okamoto等报告的多117bp;在亲缘关系上与环状病毒(Circoviridae)更近,但也可能是一个完全独立的新种。如果TT病毒基因组的确为环状,那么,它的某些分子生物学性状必定与线性病毒有较大差异,如二级结构的复杂性、滚环状复制、可重叠利用的读码框架(如同我们熟知的乙型肝炎病毒基因)等。
In 1997, Japanese scholar Nishzama and Okamoto reported for the first time that a new type of DNA virus was isolated from the serum of a post-transfusion hepatitis patient using differential display analysis (RDA), and soon reported that the DNA was single-stranded, linear, Non-enveloped, about 3739 bp in size, belonging to the Parvoviridae family, and named the TT virus. Since then, the TT virus has received widespread attention. As research on the molecular biology and epidemiology of the virus and its pathogenicity continues to deepen, various debates and different findings have emerged. Recently, it has been reported in a very persuasive way that the genome structure of the TT virus is circular and non-linear; the full-length genome is 3852 bp, 117 bp more than that reported by Okamoto et al., And the phylogenetic relationship with Circoviridae Near, but may also be a completely independent new species. If the TT virus genome is truly circular, some of its molecular biological traits must differ significantly from those of linear viruses, such as the complexity of secondary structures, rolling circle replication, and the overlapped reading frame We are familiar with hepatitis B virus genes) and so on.