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为了观察三叉神经脊束核尾侧亚核 层神经元与向臂旁核投射神经元的突触关系 ,选用 Calbindin D-2 8k和 Parvalbu-min作为标志物 ,采用 HRP逆行追踪与免疫组织化学技术相结合的双重反应技术对 层内的 Calbindin D-2 8k样和 Parvalbumin样阳性神经元与三叉神经脊束核尾侧亚核向臂旁核投射神经元之间的突触联系进行了观察。HRP注入臂旁核后 ,逆标神经元主要位于同侧三叉尾侧亚核 层至 层。Calbindin D-2 8k和 Parvalbumin样阳性胞体、纤维和终末主要集中在 层内侧带。电镜下观察 ,此二者的阳性反应产物呈弥散分布 ;两者的阳性终末与 HRP逆标神经元的胞体和树突之间主要形成轴 -树突触 ,偶见轴 -体突触 ;Calbindin D-2 8k样阳性终末与 HRP逆标神经元的树突形成的非对称性突触占 83% ,Parvalbumin样阳性终末与 HRP逆标神经元的树突形成的对称性突触占 85 %。此外 ,还观察到参与突触小球组成的初级传入纤维终末与 HRP逆标神经元的树突形成突触联系。以上结果提示 :(1)三叉神经脊束核尾侧亚核 层的 Calbindin D-2 8k样或 Parvalbumin样阳性神经元可通过突触传递机制对三叉神经脊束核尾侧亚核向臂旁核投射的神经元进行调控 ,影响外周伤害性信息向上位脑结构的传递 ;(2 )三叉神经脊束核尾侧亚核向臂旁核投?
In order to observe the synaptic relationship between the subnuclear neurons in the caudal trigeminal nucleus and the neurons in the parabrachial nucleus, Calbindin D-2 8k and Parvalbu-min were used as markers, HRP retrograde tracing and immunohistochemistry The combination of dual reaction technique was used to observe the synaptic connections between the intracellular Calbindin D-2 8k-like and Parvalbumin-like neurons and the neurons in the parabrachialis nucleus caudalis. HRP injected into the parabrachial nucleus, the anti-labeled neurons are mainly located in the ipsilateral trigeminal tail sub-nuclear layer to layer. Calbindin D-2 8k and Parvalbumin-like positive soma, fibers and terminals are mainly concentrated in the medial layer. Electron microscopy showed that the positive reaction products of the two were diffusely distributed. The positive terminal between the positive terminal of HRP and the dendrites of HRP-labeled neurons mainly formed ax-dendrites and occasionally axonal synapses. Asymmetric synapses formed by dendrites of Calbindin D-2 8k-like terminals and HRP reversed-labeled neurons accounted for 83% of the synapses formed by Parvalbumin-like positive terminals and dendrites of HRP-reversed neurons 85%. In addition, it was also observed that the primary afferent fibers involved in the formation of synaptic spheroids formed synaptic connections with the dendrites of HRP retroplied neurons. The above results suggest that: (1) Calbindin D-2 8k-like or Parvalbumin-like positive neurons in the subnuclear layer of the trigeminal nucleus caudatum can induce synaptic transmission to the parabrachial nucleus caudalis Projection of neurons to regulate the impact of peripheral damage to the upper brain structure of the transmission of information; (2) trigeminal nerve bundle nucleus caudal subnuclear parathyroid cast;