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运用限制性片段长度多态性聚合酶链反应(PCR-RFLP)检测184例T2DM患者,其中110例为DN者[DN(+)组]、74例为无肾病者[DN(-)组]及56例正常对照组(NC组)的AT1R基因多态性。结果(1)昆明汉族正常人群中AT1R基因型AA型86%,AC型14%,CC型未检出;A等位基因频率93%,C等位基因频率7%。(2)T2DM组与NC组A1R基因T的A1166C多态基因型频率和等位基因频率分布均无差异(P>0.05)。(3)DN(+)与DN(-)中AT1R基因的A1166C多态基因型频率和等位基因频率分布均无差异(P>0.05)。结论(1)昆明地区汉族正常人群AT1R基因多态性频率分布具有一定的地区特征。(2)昆明汉族T2DM及2型DN与AT1R基因A1166C多态性无关。
184 patients with T2DM were detected by restriction fragment length polymorphism (PCR-RFLP), including 110 DN patients [DN (+) group] and 74 patients without DN [DN (-) group] And 56 normal control group (NC group) AT1R gene polymorphism. Results (1) The AT1R genotype AA 86%, AC 14% of the normal population of Kunming Han nationality, CC type was not detected; A allele frequency 93%, C allele frequency 7%. (2) There was no difference in A1166C polymorphism frequency and allele frequency distribution between A1DM and T2DM groups (P> 0.05). (3) The A1166C polymorphism of AT1R gene in DN (+) and DN (-) did not differ from that of allele (P> 0.05). Conclusions (1) The frequency distribution of AT1R gene in Han normal population in Kunming has certain regional characteristics. (2) There was no correlation between T2DM and type 2 DN of Kunming Han nationality and A1166C polymorphism of AT1R gene.