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目的对北京市昌平区中国疾病预防控制中心园区内刺猬携带的中华革蜱进行斑点热群立克次体(SFGR)的分离和鉴定。方法采用形态学和分子生物学方法对SFGR进行鉴定,并进行系统进化特征分析。结果 SFGR对DH82细胞产生明显细胞病变;Gimenez染色后,可见较多清晰的立克次体被染成紫红色;细菌16S基因测定后,西伯利亚立克次体246株和西伯利亚立克次体BJ-90株相应核苷酸序列的同源性均为100%;系统进化分析显示,SFGR位于西伯利亚立克次体进化分支内,确认其为西伯利亚立克次体。结论北京市昌平区存在西伯利亚立克次体,该地区存在北亚热自然疫源地的可能性。
Objective To isolate and identify Spot Germplasm Rickettsia (SFGR) carried by hedgehog in the Park of Chinese Center for Disease Control and Prevention in Changping District of Beijing. Methods Morphology and molecular biology methods were used to identify SFGRs and their phylogenetic characteristics were analyzed. Results SFGR had obvious cytopathic effect on DH82 cells. After the Gimenez staining, more clear rickettsia was observed, and after bacterial 16S gene determination, 246 strains of Rickettsia sibirica and BJ- The homology of 90 corresponding nucleotide sequences was 100%. Phylogenetic analysis showed that SFGR was located in the evolutionary branch of Rickettsia sibirica and was identified as Siberian rickettsia. Conclusion The existence of Siberian Rickettsia in Changping District of Beijing may lead to the existence of natural foci of North Asia fever in this area.