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目的:研究锌、维生素C联合应用对实验性糖尿病小鼠血糖的影响。方法:将四氧嘧啶按200μg/mg剂量一次性腹腔注射制作糖尿病小鼠模型。实验分为4组:正常对照组、阳性对照组、补锌组、锌-维生素C联合组。锌、维生素C按2.31、16.67 mg/kg.d的量给予补充,实验组小鼠自由饮用相应浓度的Zn2+应用液和Zn2+-维生素C混合应用液。正常对照组、阳性对照组小鼠自由饮用蒸馏水。实验周期为4周,葡萄糖氧化酶法测定空腹血糖。结果:与正常对照组比较,3组糖尿病小鼠摄食量、饮水量均增加(P<0.01),且体重增长值在实验前两周明显减少(P<0.01);与阳性对照组比较,两实验组血糖水平明显降低(P<0.01),补锌组摄食量增多(前两周,P<0.01;后两周,P<0.05);锌-维生素C联合组摄食量少于补锌组(前两周,P<0.01;后两周,P<0.05)。结论:锌对糖尿病小鼠糖代谢有一定的调节作用;锌、维生素C联合应用降低糖尿病小鼠血糖的效果优于单独补锌。
Objective: To study the effects of combination of zinc and vitamin C on blood glucose in experimental diabetic mice. Methods: Alloxan was injected intraperitoneally at a dose of 200 μg / mg to make a diabetic mouse model. The experiment was divided into 4 groups: normal control group, positive control group, zinc supplement group, zinc-vitamin C combination group. Zinc and Vitamin C were supplemented by 2.31 and 16.67 mg / kg.d respectively. The mice in the experimental group were free to drink the corresponding mixed solution of Zn2 + and Zn2 + - Vitamin C. Normal control group, positive control mice were free to drink distilled water. The experimental period was 4 weeks, fasting blood glucose was measured by glucose oxidase method. Results: Compared with the normal control group, the food intake and water intake in the three diabetic groups increased (P <0.01), and the weight gain decreased significantly two weeks before the experiment (P <0.01). Compared with the positive control group, (P <0.01 for the first two weeks; P <0.05 for the second two weeks); and the dietary intake of the zinc-vitamin C combination group was less than that of the zinc supplementation group (P <0.01) P <0.01 two weeks before, P <0.05 two weeks later). Conclusion: Zinc can regulate glucose metabolism in diabetic mice. The combination of zinc and vitamin C can reduce blood glucose in diabetic mice better than that of zinc alone.