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以不同耐旱性的2个大豆品种(高耐旱JP-6、低耐旱JP-16)为研究材料,采用高效液相色谱和实时荧光定量PCR技术,分析不同时间持续干旱胁迫下,大豆叶片和根系中异黄酮的积累变化及关键酶基因的表达情况.结果表明:大豆根部异黄酮含量显著高于叶部,而异黄酮关键酶基因的表达量则在叶片中更高,耐旱品种JP-6根部的异黄酮积累量更大.随着干旱胁迫持续时间的增加,不同耐旱品种的异黄酮合成与积累变化规律存在显著差异:强耐旱品种JP-6的根和叶中,异黄酮积累量均呈现先下降后升高的趋势;而弱耐旱品种JP-16则相反,异黄酮积累量在不同部位中均呈现先上升后降低的趋势;除JP-6叶中C4H、4CL、IFS2等异黄酮合成上游基因外,其他不同品种、不同部位的关键酶基因表达量均随着干旱胁迫持续时间的增加,呈现先下降后上升的趋势.大豆叶片是异黄酮的主要合成部位,大豆根部也存在少量的异黄酮合成.弱耐旱大豆根部的异黄酮合成和最终积累量均较低,强耐旱品种则较高.根部异黄酮积累量高的大豆品种,其耐旱性更强.
Two soybean cultivars with different drought tolerance (high drought tolerance JP-6 and low drought tolerance JP-16) were used as materials to study the effects of drought stress on soybean The accumulation of isoflavones in leaves and roots and the expression of key enzyme genes were analyzed.The results showed that the content of isoflavones in soybean roots was significantly higher than that in leaf parts and the expression of key enzyme genes in isoflavones was higher in leaves, The accumulation of isoflavones in the roots of JP-6 was greater than that in the roots of JP-6.With the increase of the duration of drought stress, the variation of isoflavone synthesis and accumulation in different drought-tolerant cultivars were significantly different: Isoflavone accumulation showed the trend of first decreasing and then increasing; weak drought-tolerant varieties JP-16 on the contrary, the accumulation of isoflavone in different parts of the first showed an upward trend and then decreased. Except JP-6 leaf C4H, 4CL, IFS2 and other isoflavones, the expression level of key enzyme genes of other varieties and parts increased with the increase of the duration of drought stress, and then decreased firstly and then increased.The soybean leaf was the main synthesis site of isoflavones Soy bean root There is also a small amount of isoflavone. Drought soy isoflavone weak root accumulation and ultimately lower volume, higher strength is drought tolerant high isoflavone root accumulation of soybean varieties, more drought tolerance.