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目的:建立同时测定复方甲硝唑涂剂中甲硝唑和醋酸泼尼松含量的方法。方法:采用反相高效液相色谱法。色谱柱为DiamonsilC18,流动相为甲醇-水,梯度洗脱,流速为1.0ml/min,检测波长为238nm,柱温为20℃。结果:甲硝唑和醋酸泼尼松检测质量浓度线性范围分别为100.0～800.0(r=0.9997)、20.0～200.0(r=0.9998)μg/ml,平均回收率分别为101.3%(RSD=1.6%,n=6)、100.3%(RSD=1.8%,n=6)。结论:本方法准确度、精密度好,可用于含甲硝唑、醋酸泼尼松的复方制剂的质量控制。
Objective: To establish a method for simultaneous determination of metronidazole and prednisone acetate in compound metronidazole paints. Methods: Reversed-phase high performance liquid chromatography. The column was Diamonsil C18 and the mobile phase was methanol-water. The gradient elution was performed at a flow rate of 1.0 ml / min. The detection wavelength was 238 nm and the column temperature was 20 ℃. Results: The linear ranges of metronidazole and prednisone acetate were 100.0-800.0 (r = 0.9997) and 20.0-200.0 (r = 0.9998) μg / ml, respectively. The average recoveries were 101.3% (RSD = 1.6% , n = 6), 100.3% (RSD = 1.8%, n = 6). Conclusion: The method has good accuracy and precision, and can be used for quality control of compound preparations containing metronidazole and prednisone acetate.