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目的:采用双向凝胶电泳技术(2-DE),分析原发性局灶节段硬化性肾小球肾炎(FSGS)患者使用地塞米松冲击治疗前后的尿液蛋白图谱的差异及其敏感性。方法:以FSGS患者为研究对象,观察地塞米松冲击治疗前、冲击治疗后3d、冲击治疗后2周3个时间点的尿液双向电泳图谱的蛋白点差异分布,并与其同期尿液分析及24h尿蛋白定量结果相比较。结果:FSGS患者尿液样品2-DE图谱显示:地塞米松冲击治疗前与冲击后3d比较其蛋白点数量存在明显差异,但24h蛋白定量值差异无统计学意义(P>0.05);地塞米松冲击治疗前与冲击后2周比较其蛋白点数量存在明显差异且24h蛋白定量值差异有统计学意义(P<0.05)。结论:FSGS患者使用地塞米松冲击治疗后,其尿液中的蛋白点明显减少,结合其24h尿蛋白定量结果提示:尿蛋白点数减少与临床疗效具有一致性,其敏感程度较24h尿蛋白定量高。
OBJECTIVE: To analyze the difference and sensitivity of urine protein profiles before and after dexamethasone treatment in patients with primary focal segmental glomerulonephritis (FSGS) using two-dimensional gel electrophoresis (2-DE) . Methods: The FSGS patients were studied. The difference of the protein spots in urine two-dimensional electrophoresis before and after dexamethasone treatment three days and three weeks after shock treatment were observed. 24h urinary protein quantitative results compared. Results: The 2-DE map of urine samples from patients with FSGS showed that there was a significant difference in the number of protein spots before and after dexamethasone treatment but there was no significant difference in the 24h protein quantitation (P> 0.05) There was a significant difference in the number of protein spots between the pre-treatment and the 2-week post-challenge of mitoxantrose treatment, and the difference was statistically significant (P <0.05). Conclusions: After dexamethasone shock treatment, the protein spots in urine of FSGS patients decreased significantly. The quantitative results of urinary protein in 24 hours showed that the decrease of urinary protein was consistent with the clinical efficacy, and the sensitivity was 24 h high.