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采用聚合酶链式反应（PCR法）对73例结核病患者和60例非结核病患者不同部位的标本进行检测，同时进行涂片法抗酸染色。BACTEC460TB系统结核菌培养，分别比较其阳性率与特异性。结果发现73例不同部位结核性标本中PCR法、涂片法、培养法总阳性率分别为47．9％、21．9％、35．7％，PCR法与其它两种方法比较有显著性差异（P<0．05）。60例非结核性标本（阴性）特异性分别为76．7％、100％、96．7％，PCR法与其它两种方法比较有显著性差异（P＜0．05）。
73 cases of tuberculosis patients and 60 cases of non-tuberculosis patients in different parts of the specimens were detected by polymerase chain reaction (PCR), smear method simultaneously acid-fast staining. BACTEC460TB system TB culture, respectively, compared with the positive rate and specificity. The results showed that the total positive rates of PCR, smear and culture in 73 specimens of tuberculosis in different sites were 47.9%, 21.9% and 35.7% respectively. There was significant difference between PCR and other two methods Difference (P <0.05). The specificity of 60 cases of non-tuberculous specimens (negative) were 76.7%, 100% and 96.7% respectively. There was significant difference between PCR and other two methods (P <0.05).