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目的:探讨蔷薇红景天的抗氧化作用。方法:通过大鼠肝微粒体的提取,制备了Fe2+-半胱氨酸诱导的脂质过氧化损伤模型,检测蔷薇红景天不同提取分离部位的样品对丙二醛(MDA)抑制作用的影响,观察蔷薇红景天的抗氧化作用。结果:在Fe2+-半胱氨酸诱导的微粒体脂质过氧化模型中,乙酸乙酯不溶层浸膏样品(X6)、大孔树脂30%乙醇分离浸膏样品(X11)、大孔树脂50%乙醇分离浸膏样品(X12)、大孔树脂70%乙醇分离浸膏样品(X13)、大孔树脂Sephadex甲醇洗脱浸膏样品(X16)对MDA抑制率和IC50值均大于50%,并且反应浓度在100μg/mL,和50μg/mL时,样品对MDA抑制作用明显。结论:提示蔷薇红景天具有抗氧化作用。
Objective: To explore the anti-oxidation effects of Rhodiola rosea. METHODS: Fe2+-cysteine-induced lipid peroxidation injury model was established by the extraction of rat liver microsomes. The effect of different extracts from Rhodiola rosea on the inhibition of malondialdehyde (MDA) was tested. Observe the antioxidant effects of Rhodiola rosea. Results: In the Fe2+-cysteine-induced microsomal lipid peroxidation model, ethyl acetate insoluble layer extract sample (X6), macroporous resin 30% ethanol separation extract sample (X11), macroporous resin 50 Ethanol separation extract samples (X12), macroporous resin 70% ethanol separation extract samples (X13), and macroporous resin Sephadex methanol elution extract samples (X16) had greater than 50% MDA inhibition rate and IC50 value, and When the reaction concentration was 100 μg/mL and 50 μg/mL, the inhibitory effect of the sample on MDA was significant. Conclusion: It is suggested that Rhodiola rosea has antioxidant effect.