论文部分内容阅读
目的:评价人类冷冻胚胎解冻后,在胚胎移植前,采用激光辅助孵化及吸出胚胎的坏死细胞碎片是否会提高胚胎的种植率。方法:回顾性的分析了91个冷冻胚胎移植周期的临床妊娠率和胚胎植入率,其中4 1个周期(A组)在胚胎移植前,采用激光辅助孵化结合显微操作去除冷冻胚胎中的坏死细胞碎片,5 0个周期(B组) ,采用激光辅助孵化,但没有去除坏死细胞碎片。采用慢速冷冻 快速解冻的方法。结果:在冷冻胚胎移植前去除胚胎的坏死细胞碎片,能显著性地提高移植周期的临床妊娠率(43.91%vs.2 4 .0 0 % ,P <0 .0 5 )和胚胎植入率(19.4 5 %vs.10 30 % ,P <0 .0 5 )。结论:在人类冷冻胚胎移植前,采用激光及显微操作辅助去除冷冻胚胎的坏死细胞碎片,能有效提高胚胎的种植率。
OBJECTIVES: To evaluate whether laser-assisted hatching and aspiration of necrotic cell debris from embryos before embryo transfer can improve embryo implantation rates following thawing of human frozen embryos. Methods: The clinical pregnancy rates and embryo implantation rates of 91 frozen embryo transfer cycles were retrospectively analyzed. Among 41 cycles (group A), laser-assisted hatching and micromanipulation were used to remove frozen embryos before embryo transfer Necrotic cell debris, 50 cycles (group B), laser-assisted hatching but did not remove necrotic cell debris. Slow thawing using rapid freezing method. Results: The removal of necrotic cell debris from embryos before frozen embryo transfer significantly improved the clinical pregnancy rate (43.91% vs.2 4 .0 0%, P <0 05) and embryo implantation rate ( 19.4 5% vs. 10 30%, P <0.05). Conclusion: Before human frozen embryo transfer, laser and micromanipulation can be used to remove necrotic cell debris of frozen embryo, which can effectively improve the implantation rate of embryo.