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目的:揭示脑微血管内皮细胞生理、病理及通络中药处理后不同状态的培养液对正常星形胶质细胞影响的特征,从细胞间相互作用角度探讨脑微血管内皮细胞与星形胶质细胞的生物学关系,为阐释脑微环境稳定的血脑屏障维护机制以及通络中药通过内皮细胞调节脑内微环境理论假说提供新的证据。方法:制备正常、拟缺血和拟缺血合并通络救脑注射液处理的大鼠脑微血管内皮细胞条件培养液,观察其对星形胶质细胞活性和凋亡率的影响。结果:与正常星形胶质细胞相比,正常内皮细胞条件培养液能够降低正常星形胶质细胞的活性,并促进星形胶质细胞的凋亡;而拟缺血处理的内皮细胞条件培养液能够提高正常星形胶质细胞的活性和凋亡率;拟缺血合并通络药物处理的内皮细胞条件培养液对正常星形胶质细胞的活性有提高作用,并显著降低其凋亡率。结论:三种不同处理方式的内皮细胞条件培养液对正常星形胶质细胞活性和凋亡产生不同的影响,提示不同状态的微血管内皮细胞对脑内微环境产生影响,通络救脑注射液可能通过调节微血管内皮细胞的分泌而对星形胶质细胞发挥作用。
OBJECTIVE: To reveal the effects of different culture media of brain microvascular endothelial cells on normal astrocytes after their physiological and pathological characteristics, and to explore the effects of different culture media of different states on the astrocytes of human brain microvascular endothelial cells Biological relationships to provide new evidence for elucidating the mechanism of maintenance of blood-brain barrier in the brain microenvironment and the theoretical hypothesis that Tongluo Chinese herbs regulate brain microenvironment through endothelial cells. Methods: The conditioned medium of rat brain microvascular endothelial cells treated with Tongjiu Yaonan injection and normal, ischemic and ischemic preconditioning were prepared. The effects on astrocyte activity and apoptosis rate were observed. RESULTS: Compared with normal astrocytes, normal endothelial cell conditioned medium decreased the activity of normal astrocytes and promoted the apoptosis of astrocytes, while the conditioned medium of ischemic cells Liquid can improve the activity and apoptosis rate of normal astrocytes. The conditioned medium containing hypoxic ischemic-reperfused drug can enhance the activity of normal astrocytes and significantly reduce the apoptosis rate . CONCLUSION: The three different treatment methods of endothelial cell conditioned medium have different effects on normal astrocyte activity and apoptosis, suggesting that different states of microvascular endothelial cells have an impact on brain microenvironment. Tongluo Jourao injection It may play a role in astrocytes by regulating the secretion of microvascular endothelial cells.