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2002~2004年浙江省发生了无菌性脑膜炎暴发疫情,为了及时查明病因,分析病原的分子特征并进行病原溯源,我们采集患者脑脊液和粪便样本271份,用RD和Hep-2细胞同时分离病毒,对分离株VP1和VP4/VP2基因测序,进行同源性与进化分析。结果从271份样本中分离到埃柯病毒30型(E30)78株;对31株分离株VP1区核苷酸(nt)序列测定,其长度均为876nt,推导编码292个氨基酸(aa)。浙江E30株与原型株Bastianni在VP1区的nt和aa同源性分别为84.7%~86.3%和92.1%~94.2%;浙江E30株之间nt和aa的同源性分别为87.1%~99.4%和96.2%~100%。在VP1基因进化树上浙江E30株分别位于G和H基因亚型分支上,与浙江E30G亚型株亲缘关系最近的国内外毒株分别为2003年江苏、山东株和1999年乌克兰株;与浙江E30H亚型株亲缘关系最近的毒株为2008年韩国株。VP4/VP2区同源性与进化分析结果与VP1相似。结果表明2002~2004年浙江省无菌性脑膜炎暴发疫情由E30G和H二类不同基因亚型流行株引起;H基因亚型株推测为新的E30变异株,首先分离于2002年浙江省。
Aseptic meningitis outbreak occurred in Zhejiang Province from 2002 to 2004. In order to identify the cause of the disease in time and analyze the molecular characteristics of the pathogen and trace the origin of the pathogen, we collected 271 samples of cerebrospinal fluid and stool samples from patients with RD and Hep-2 cells simultaneously Viruses were isolated and the isolates VP1 and VP4 / VP2 genes were sequenced for homology and evolution analysis. Results 78 isolates of E30 (E30) were isolated from 271 samples. The nucleotide sequence of VP1 of all 31 isolates was 876 nt in length and deduced 292 amino acids (aa). The nt and aa homologies between Zhejiang E30 and Bastianni in VP1 were 84.7% -86.3% and 92.1% -94.2%, respectively. The homologies of nt and aa in Zhejiang E30 were 87.1% -99.4% And 96.2% ~ 100%. Zhejiang strain E30 was located on the branch of G and H subtypes in the VP1 gene phylogenetic tree. The domestic and foreign strains with the closest genetic relationship with Zhejiang E30G subtype were Jiangsu and Shandong strains in 2003 and Ukraine strain in 1999 respectively. The closest strain of the E30H subtype was the Korean strain of 2008. VP4 / VP2 region homology and evolution analysis results and VP1 similar. The results showed that the outbreak of aseptic meningitis in Zhejiang Province from 2002 to 2004 was caused by two subtypes of E30G and H genotypes. The H subtype was presumed to be a new E30 mutant, which was isolated in Zhejiang Province in 2002.