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目的观察外周血T淋巴细胞亚群在2型糖尿病(T2DM)患者及大血管病变患者的变化,探讨其在T2DM及大血管病变发病中的作用。方法应用免疫荧光标记技术和流式细胞仪,检测36例初诊T2DM(单纯T2DM组)患者、28例T2DM并发大血管病变患者及20名健康对照者的外周血T细胞亚群变化。结果T2DM并发大血管病变组、单纯T2DM组CD4+T细胞、CD4/CD8比值、CD4+CD28-T细胞明显高于健康对照组(均P<0.05);而CD3、CD8+T细胞、CD4+CD25+T细胞明显低于健康对照组(均P<0.05);T2DM并发大血管病变组CD4+T细胞、CD4/CD8比值、CD4+CD28-T细胞明显高于单纯T2DM组(P<0.05),CD3、CD8+T细胞、CD4+CD25+T细胞低于单纯T2DM组。结论机体细胞免疫异常在T2DM及大血管病变的发生、发展中发挥着一定的作用。
Objective To observe the changes of peripheral blood T lymphocyte subsets in patients with type 2 diabetes mellitus (T2DM) and macrovascular diseases and to explore its role in the pathogenesis of T2DM and macroangiopathy. Methods The changes of T lymphocyte subsets in peripheral blood of 36 newly diagnosed T2DM patients (T2DM group), 28 T2DM patients with macrovascular disease and 20 healthy controls were detected by immunofluorescence labeling and flow cytometry. Results The percentage of CD4 + T cells, CD4 / CD8 and CD4 + CD28-T cells in T2DM complicated with macroangiopathy group and T2DM group were significantly higher than those in healthy control group (all P <0.05); while CD3, CD8 + T cells, CD4 + CD25 + T cells in T2DM group were significantly lower than those in T2DM group (all P <0.05); CD4 + T cells, CD4 / CD8 ratio and CD4 + CD28- , CD3, CD8 + T cells and CD4 + CD25 + T cells were lower than those in T2DM alone. Conclusion The abnormalities of cellular immunity play an important role in the occurrence and development of T2DM and macroangiopathy.