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目的探讨张家口地区卵巢癌易感性与CYP1A1基因MspI位点多态性的关系。方法研究采用PCR-RFLP技术,将张家口地区34例卵巢癌患者作为实验组,45例健康女性人群作为对照组,分析CYP1A1基因3′端限制性内切酶MspI位点基因的基因多态性。结果卵巢癌组MspI基因型分布为:基因型TT占21.8%;基因型TC占52.7%;基因型CC占25.5%;等位基因T、C分别为48.2%、51.8%。健康人群组MspI基因型分布为,基因型TT占42.2%;基因型TC占46.7%;基因型CC占11.1%;等位基因T、C分别为65.6%、34.4%。结论该地区健康人群组和卵巢癌组CYP1A1基因MspI位点均呈多态性分布,两者之间差异有显著性(P<0.05),提示卵巢癌发病率可能与CYP1A1基因型有关。
Objective To investigate the relationship between the susceptibility of ovarian cancer and the MspI polymorphism of CYP1A1 gene in Zhangjiakou area. Methods Using PCR-RFLP technique, 34 ovarian cancer patients in Zhangjiakou were selected as the experimental group and 45 healthy female population as the control group. The gene polymorphism of the 3 ’end restriction endonuclease MspI gene of CYP1A1 gene was analyzed. Results The genotypes of MspI in ovarian cancer group were 21.8% for genotype TT, 52.7% for genotype TC, 25.5% for genotype CC and 48.2% and 51.8% for allele T and C, respectively. The distribution of MspI genotype in healthy population was 42.2% for genotype TT, 46.7% for genotype TC, 11.1% for genotype CC, and 65.6% and 34.4% for allele T and C, respectively. Conclusion The MspI loci of CYP1A1 gene in healthy population and ovarian cancer patients in this area showed polymorphism distribution (P <0.05), suggesting that the incidence of ovarian cancer may be related to CYP1A1 genotype.