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以辣椒品种“湘研16号”为试材,采用人工气候箱模拟低温环境,研究了2,4-表油菜素内酯(EBR)对低温胁迫下辣椒幼苗体内超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)活性以及抗坏血酸(As A)和谷胱甘肽(GSH)含量的影响,以期为油菜素内酯调控辣椒幼苗耐低温胁迫的机理研究提供参考依据。结果表明:抗氧化酶(SOD、POD、CAT、APX)活性在低温胁迫下高于CK,而EBR处理的叶片酶活性显著增加。同时,EBR提高了低温胁迫下辣椒幼苗叶片中抗坏血酸(As A)和谷胱甘肽(GSH)含量,降低了MDA含量。由此可见,EBR能显著增加保护酶活性和非酶抗氧化物的含量,增强植株抗氧化能力,有效缓解低温胁迫引起的膜脂过氧化伤害,降低了低温胁迫对辣椒幼苗生长的抑制作用。
In this experiment, artificial climate chamber was used to simulate the low temperature environment with pepper cultivar “Xiangyan 16”, and the effects of 2,4-epibrassinolide (EBR) on the activity of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX) and the content of As A and GSH were studied in order to study the effects of brassinolide Lactone regulation of pepper seedling mechanism of low temperature stress provide a reference. The results showed that the activity of antioxidant enzymes (SOD, POD, CAT, APX) was higher than that of CK under low temperature stress, while the enzyme activity of EBR treated leaves increased significantly. At the same time, EBR increased the content of As A and GSH in the leaves of pepper seedlings and decreased the content of MDA. Thus, EBR can significantly increase the content of protective enzyme activity and non-enzyme antioxidants, enhance the antioxidant capacity of plants, effectively alleviate the damage caused by membrane lipid peroxidation caused by low temperature stress, and reduce the inhibitory effect of low temperature stress on the growth of pepper seedlings.