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为了寻找简便快速地检测抗-HAVIgM的方法,采用实验室实验的方法,将抗人μ链单克隆抗体包被于硝酸纤维素膜(NCM),胶体金标记抗-HAVIgG抗体,以细胞培养的甲肝抗原作为标本中抗-HAVIgM与金标记抗-HAVIgG的联接剂,建立了捕获式滴金免疫检测法。结果显示,该法与酶联免疫法对比测定,两者总符合率为94%。包被抗体的NCM和金标抗体在4℃下至少可保存3个月。该法简便、快速,整个操作过程仅需几分钟。适于各级医疗单位用作甲型肝炎的早期诊断和流行病学监测
In order to find a simple and rapid method for detecting anti-HAVIgM, anti-human μ-chain monoclonal antibody was coated on nitrocellulose membrane (NCM) and colloidal gold labeled anti-HAV IgG in a laboratory experiment. Cultured hepatitis A antigen as a specimen of anti-HAVIgM and gold labeled anti-HAV Igg coupling agent, established capture gold droplet immunoassay. The results show that the method and enzyme-linked immunosorbent assay, the total coincidence rate was 94%. NCM and gold-labeled antibodies coated with antibodies can be stored for at least 3 months at 4 ° C. The method is simple, fast and requires only a few minutes for the entire operation. Suitable for all levels of medical units for the early diagnosis of hepatitis A and epidemiological surveillance