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目的:采用鼠伤寒沙门菌回复突变(AMES)试验和哺乳动物微核试验对崖柏精油的遗传毒性进行评价。方法:AMES试验采用TA97、TA98、TA100和TA102四种菌株,对崖柏精油的致突变性进行评价。采用小鼠骨髓噬多染红细胞微核试验,对本品的染色体毒性进行评价。结果:崖柏精油不同剂量组对TA97、TA98、TA100、TA102四种试验菌株,在加大鼠肝微粒体酶S9和不加S9的情况下,回复突变数均不超过溶剂对照组的1倍,差异无统计学意义(P<0.05),为诱变阴性。微核试验表明,崖柏精油各剂量组对小鼠骨髓微核率无显著影响(P>0.05)。结论:崖柏精油无明显遗传毒性。
OBJECTIVE: To evaluate the genotoxicity of essential oils of Thuja spp. Using Salmonella typhimurium (AMES) test and mammalian micronucleus test. Methods: AMES test TA97, TA98, TA100 and TA102 four strains of essential oils of Thuja essential oil mutagenicity were evaluated. Using mouse bone marrow polychromatic erythrocyte micronucleus test to evaluate the chromosomal toxicity of this product. Results: There was no significant difference in the numbers of back mutations among the tested strains of TA97, TA98, TA100 and TA102 in the different dose groups of Acorus calamus essential oil with and without S9 and S9 , The difference was not statistically significant (P <0.05), as mutagenic negative. The results of micronucleus test showed that there was no significant effect on the micronuclei frequency of mice bone marrow in each dose group (P> 0.05). Conclusion: Thuja essential oil has no obvious genotoxicity.