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以茄子品种‘禾线’为材料,采用同源克隆方法从茄子中克隆获得一个MnSOD基因,其开放阅读框全长为687 bp,编码228个氨基酸,将其命名为SmMnSOD。序列分析表明,SmMnSOD与辣椒MnSOD序列相似性达到91%,与番茄MnSOD序列相似性达到84%。预测该蛋白质分子量为25.63 k Da,蛋白等电点为8.46。亚细胞定位结果显示,MnSOD蛋白定位于线粒体。实时荧光定量PCR表明,SmMnSOD在不同组织器官中均有表达,但在叶片中表达量最多,其次为花瓣和根,果肉中最少。在Na Cl胁迫下,SmMnSOD表达量呈现先上升后下降趋势;聚乙二醇胁迫抑制了SmMnSOD的表达;外源脱落酸胁迫下,SmMnSOD的表达量是波动变化的;低温胁迫极显著地抑制了SmMnSOD的表达。实验结果证明,SmMnSOD主要在茄子叶片、花瓣和根中表达,在线粒体中发挥功效,可能与茄子抵御渗透性胁迫和干旱胁迫相关。
An eggplant variety ’Wo Line’ was used to clone a MnSOD gene from eggplant using homologous cloning method. The open reading frame of the MnSOD gene was 687 bp in length and encoded 228 amino acids, which was named as SmMnSOD. Sequence analysis showed that the similarity of SmMnSOD with pepper MnSOD sequence was 91%, and that of tomato MnSOD sequence was 84%. The molecular weight of the protein was predicted to be 25.63 kDa and the isoelectric point of the protein was 8.46. Subcellular localization results showed that MnSOD protein localized to mitochondria. Real-time fluorescence quantitative PCR showed that SmMnSOD was expressed in different tissues and organs, but it was the most abundant in leaves, followed by petals and roots, with the least in the flesh. Under NaCl stress, the expression level of SmMnSOD firstly increased and then decreased, while that of polyethylene glycol inhibited the expression of SmMnSOD. The expression level of SmMnSOD fluctuated under the exogenous ABA stress. SmMnSOD expression. The experimental results show that SmMnSOD is mainly expressed in the leaves, petals and roots of eggplant and plays an important role in mitochondria, which may be related to the resistance of eggplant against osmotic stress and drought stress.