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目的研究溺死猪肺内不同部位和左、右心室血的硅藻含量、长径和种类差异,为硅藻检验送检提供参考依据。方法 32只五指山猪随机分为陆地死亡组(n=2)、死后入水组(n=15)和溺死组(n=15);采用微波消解-扫描电镜法对各肺叶边缘和中部组织及左、右心室血内的硅藻进行定性和定量分析。结果死后入水组肺内硅藻含量小于溺死组各肺叶内硅藻含量(P<0.05);溺死组各肺叶之间硅藻的含量及长径无显著差异,各肺叶均以小环藻、菱形藻、直链藻为主。溺死组左心室血硅藻含量高于右心室血(P<0.05);左、右心室血内硅藻长径差异无统计学意义;溺液中8种硅藻可在左心室血内检出,右心室血内仅检出含量高的4种硅藻。结论肺组织的硅藻检验推荐取材右肺膈叶或左、右尖叶的中部组织;心血推荐取材左心室血,并选用灵敏度高的MD-SEM法可提高硅藻检验阳性率和检出种类。
Objective To study the differences in diatom content, long diameter and species in different parts of the lungs and left and right ventricular blood of drowning pigs, and provide a reference for the diatom inspection. Methods Twenty-two Wuzhishan pigs were randomly divided into land death group (n = 2), post-mortem water group (n = 15) and drowning group (n = 15). Microwave digestion-scanning electron microscopy Diatoms in the left and right ventricular blood were qualitatively and quantitatively analyzed. Results The content of diatoms in the lungs of the infants after death was less than that of the diatoms in the lungs of the drowning group (P <0.05). There was no significant difference in the content and the long diameter of diatoms among the lobes of the drowning group. Diamond-shaped algae, mainly straight-chain algae. The content of diatoms in the left ventricular blood of the drowning group was higher than that of the right ventricle (P <0.05), while there was no significant difference in the diameters of the diatoms between the left and right ventricles. The eight diatoms in the drowning fluid were detected in the left ventricular blood Only 4 kinds of diatoms were detected in the right ventricle blood. Conclusion Diatom test in lung tissue is recommended to take the right lung diaphragm or the left and right tip of the middle of the organization; recommended blood drawn from the left ventricular blood, and the choice of high sensitivity MD-SEM method to improve diatom test positive rate and detection of species .