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目的:检测人类染色体端粒酶基因(h TERC)在低度宫颈上皮内瘤样病变(CIN)Ⅰ中的表达情况,并进一步探讨h TERC基因在预测CINⅠ向高级别宫颈上皮内瘤样病变进展的价值。方法:选取2010年1月-2010年12月佛山市妇幼保健院病理活检确诊为CINⅠ的病例110例,应用荧光原位杂交技术(FISH)检测110例患者宫颈脱落细胞中h TERC基因的扩增情况,根据扩增结果将患者分为观察组与对照组,进行复查。结果:观察组与对照组相比较,进展为CINⅡ或CINⅢ的发生率显著高于对照组,差异有统计学意义(P<0.05),转归为正常及仍保持CINⅠ的几率显著低于对照组,差异有统计学意义(P<0.05)。结论:h TERC基因在CINⅠ的进展过程中可能起重要的作用,CINⅠ患者h TERC基因的表达情况有望可以用作预测其发展到更高级别上皮内瘤样病变的良好指标。
OBJECTIVE: To detect the expression of human telomerase reverse transcriptase (hTERC) in low grade cervical intraepithelial neoplasia (CIN) Ⅰ and to further explore the role of h TERC in predicting the progression from CINⅠ to high grade cervical intraepithelial neoplasia the value of. Methods: From January 2010 to December 2010, 110 cases of CINⅠ confirmed by pathological biopsy in Foshan MCH hospital were enrolled. The amplification of h TERC gene in 110 cases of cervical exfoliated cells was detected by fluorescence in situ hybridization (FISH) According to the amplification results, the patients were divided into observation group and control group for review. Results: Compared with the control group, the incidence of CINⅡ or CINⅢ in the observation group was significantly higher than that in the control group, the difference was statistically significant (P <0.05), the chance of returning to normal and still maintaining CINⅠwere significantly lower than that of the control group , The difference was statistically significant (P <0.05). CONCLUSION: h TERC gene may play an important role in the progression of CIN I. The expression of h TERC gene in CIN I patients is expected to be a good predictor of its development to higher grade intraepithelial neoplasia.