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：HeLa细胞KB株、X株、NM20／X株、H株的染色体众数依次为60±3（超二倍体）、62±3（超二倍体）、68±3（超二倍体和亚四倍体）和78±2（亚四倍体），所占比率分别为72%～76%，69%，52%和40%。在纯化3代的肿瘤阴性对照二倍体猫肾（染色体众数38所占比率80%）和犬肾原代细胞皮下接种裸鼠的致癌／致瘤率分别为0%（0／22）和0%（0／10），X株HeLa细胞冻融裂解物皮下接种裸鼠产生进行性缩小肿瘤的比率为20%（1／5）的前提下，HeLa细胞KB株、X株、NM20／X株皮下接种裸鼠产生进行性生长恶性肿瘤的比率分别为100%（10／10），100%（25／25）和100%（5／5），H株细胞皮下接种裸鼠产生恶性肿瘤的比率为50%（5／10）。其中，只有HeLa细胞KB株10～11代（染色体结构畸变率高达20%，出现18%双着丝点和2%断片）以超高数量接种的1组4只裸鼠（0.17ml 12.75×107／鼠）才均形成MRT，特别是从染色体众数不同的3株HeLa细胞中筛选出致瘤性强的X株，连传20代后皮下接种裸鼠形成低分化肿瘤，定名为NM20／X株0代，其染色体众数因经裸鼠体内传代选育而明显增大（染色体众数为68±3，所占比率为52%），实体瘤手术切除后体外连传11代，再皮下接种裸鼠均形成MRT（5／5），但要求完形活细胞接种量要大（5～12×107／鼠），肿瘤产生?
: Chromosome population of HeLa cells KB strain, X strain, NM20/X strain, and H strain was 60±3 (superdiploid), 62±3 (superdiploid), and 68±3 (superdiploid) And subtetraploid) and 78 ± 2 (subtetraploid), the proportions were 72% to 76%, 69%, 52% and 40%, respectively. The oncogenic/oncogenic rates of nude mice that were subcutaneously inoculated with canine kidney primary cells were 0% (0/22) for the three generations of tumor-negative control diploid feline kidneys (80% of the chromosomes with a mode frequency of 38) and were purified. 0% (0/10), HeLa cells KB strain, X strain, NM20/X under the premise of 20% (1/5) subcutaneous inoculation of X-cell HeLa cell freeze-thaw lysate into nude mice to produce progressive tumor shrinkage Subcutaneous inoculation of nude mice resulted in 100% (10/10), 100% (25/25) and 100% (5/5) malignant tumors. Nude mice were inoculated subcutaneously with H cells to produce malignant tumors. The ratio is 50% (5/10). Among them, only HeLa cell line KB strains from the 10th to the 11th generation (chromo-structural distortion rate of up to 20%, 18% dicentrics and 2% fragmentation) were inoculated in an ultra-high number of 4 nude mice (0.17 ml 12.75×107 Only MRT was formed in mice and mice, especially X-strains with strong tumorigenicity were screened out from 3 strains of HeLa cells with different chromosome patterns. Twenty consecutive passages of nude mice were inoculated subcutaneously to form poorly differentiated tumors. The name was NM20/X. In the 0th generation, the number of chromosomes was significantly increased due to cross breeding in nude mice (68±3 for the chromosome number, accounting for 52%). After solid tumors were removed in vitro for 11 generations, and then subcutaneously. MRT (5/5) was formed in mice inoculated with nude mice, but a large amount of live cells (5 to 12×10 7 cells per mouse) was required for tumor formation.