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从秦皇岛某海水养殖场牙鲆(ParalichthysolivaceusL.)细菌性败血感染症的病例中,分离到了相应的病原细菌。经对12株纯培养菌进行形态特征、培养特性、理化特性等方面较系统的表观分类学指征及代表菌株DNA中G+Cmol%的测定,表明为弧菌属(VibrioPacini1854)细菌的一个新种,并定名为秦皇岛弧菌(Vibrioqinhuangdaorasp.nov.)。同时对该菌的血清型进行了检定,表明12株菌具有同种的K抗原和同种的O抗原(血清同源);另外,经以37种抗菌类药物做敏感性测定,结果显示对供试的头孢唑啉等32种药物敏感、对青霉素G等5种耐药,在不同菌株间无明显的敏感与耐药差异。
In the case of bacterial sepsis infection of Paralichthys olivaceus L. from a marine aquaculture farm in Qinhuangdao, the corresponding pathogenic bacteria were isolated. The morphological characteristics, culture characteristics, physico-chemical properties and other aspects of the systematical apparent taxonomic indications and determination of the G + C mol% of the DNA of the representative strains of 12 strains of pure cultures showed that one strain of VibrioPacini 1854 New species, and named as Vibrioqinhuangdaorasp.nov. At the same time, the serotypes of the bacteria were tested, indicating that 12 strains have the same kind of K antigen and the same type of O antigen (serum homology); In addition, the sensitivity of 37 antibacterial drugs, the results showed that Cefazolin for the test of 32 kinds of drugs sensitive to penicillin G and other five kinds of resistance, there is no significant difference between the different strains of sensitive and resistant.