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1试剂和仪器虎尿由吉林市江南公园提供东北虎虎尿。取新鲜虎尿自然沉淀1d,去沉渣留上清,用脱脂棉纱布过滤,煮沸,分装瓶中.置冰箱待用。刀豆蛋白A(ConA)和细菌脂多糖(LPS)为美国Slgma公司产品。RPMI-1640(GIBCO公司)。酶联论疫检测仪为国营华联电子管厂制造。2实验分
1 Reagents and Instruments Tiger urine A tiger tiger urine is provided by Jilin Jiangnan Park. Take a fresh tiger urine natural precipitation 1d, to leave the supernatant, use a cotton gauze filter, boil, sub-bottle. Refrigerator set aside. Concanavalin A (ConA) and bacterial lipopolysaccharide (LPS) are American Slgma products. RPMI-1640 (GIBCO Company). The enzyme linked epidemic detector is manufactured by the state-owned Hualian Electronic Tube Factory. 2 experimental points