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目的:探讨miR-301a-3p对大鼠星形胶质细胞凋亡与增殖的影响。方法:获取并培养大鼠原代星形胶质细胞,将miR-301a-3p agomir以及antagomir分别转染至细胞。分Blank组、miR-NC对照组、miR-301a组、antagomir组,每组设3个复孔,每孔2×10n 5个细胞,CCK8实验检测各组细胞的增殖能力变化;用流式细胞术及Caspase-3活性检测分析细胞凋亡的情况。n 结果:与Blank组(48 h:0.83±0.09;72 h:1.20±0.21;96 h:1.65±0.17)和miR-NC组(48 h:0.79±0.10;72 h:1.12±0.25;96 h:1.60±0.15)相比,miR-301a组(48 h:1.16±0.07;72 h :1.56±0.11;96 h:2.13±0.14)细胞的增殖能力显著提高(n P<0.05),miR-301a组细胞的凋亡率显著下降(Blank组:10.44±1.33,miR-NC组:9.84±1.40,miR-301a组:4.32±0.51,n P<0.05);而antagomir组(48 h:0.52±0.12;72 h:0.72±0.09;96 h:1.01±0.15)细胞转染后增殖能力较Blank组和miR-NC组显著降低(n P<0.05),antagomir组细胞的凋亡率显著升高(Blank组:10.44±1.33,miR-NC组:9.84±1.40,antagomir组:21.41±2.57,n P<0.05)。n 结论:miRNA-301a-3p过表达促进大鼠星形胶质细胞的增殖,抑制细胞的凋亡通路,降低细胞的凋亡,从而调控大鼠星形胶质细胞的生物学功能。“,”Objective:To explore the effects of miR-301a-3p on proliferation and apoptosis of astrocytes in rats.Methods:miR-301a-3p agomir and miR-301a-3p antagomir were synthetized and transfected into astrocytes. The cells were divided into Blank group, miR-NC group, miR-301a agomir group and antagomir group.Each group has 3 multiple pores, 2×10n 5 cells per pore. CCK8 method was used to detect proliferation and growth ability of astrocytes in each group. Anncxin V-FITC/PI cytometry and Caspase-3 were used to test apoptosis of cells in each group.n Results:Compared with Blank group (48 h: 0.83±0.09; 72 h: 1.20±0.21; 96 h: 1.65±0.17) and miR-NC group (48 h: 0.79±0.10; 72 h: 1.12±0.25; 96 h: 1.60±0.15), the proliferation ability of miR-301a group (48 h: 1.16±0.07; 72 h: 1.56±0.11; 96 h: 2.13±0.14) was significantly improved (n P<0.05), and the apoptosis rate of miR-301a group decreased significantly (Blank group: 10.44±1.33, miR-NC group: 9.84±1.40, miR-301a group: 4.32±0.51,n P<0.05). Compared with Blank group and miR-NC group, the proliferation ability of the cells in antagomir group (48 h: 0.52±0.12; 72 h: 0.72±0.09; 96 h: 1.01±0.15) decreased significantly (n P<0.05), and the apoptotic rate was significantly increased in the antiagor group (Blank group: 10.44±1.33, miR-NC group: 9.84±1.40, antiagor group: 21.41±2.57,n P<0.05).n Conclusion:miRNA-301a-3p hyperexpression can promote the proliferation of astrocytes and inhibit the apoptosis pathway, thereby regulating the biological function of rat astrocytes.