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目的 研究 Cyclin D1 ,p16在卵巢上皮性癌发生过程中的表达及相关性 .方法 采用免疫组织化学 SABC法检测卵巢上皮性癌 ,交界性、良性卵巢肿瘤及正常卵巢组织 Cy-clin D1 ,p16的表达 .结果 Cyclin D1 表达具有明显趋势性 ,恶性肿瘤组最高 (49/84,5 8.3% ) ,随病情进展 (正常卵巢组织 -良性肿瘤 -交界性肿瘤 -上皮性癌 ) ,表达呈上升趋势 (分别为 10 .0 % ;2 6 .1% ;45 .0 % ;5 8.3% ) ,恶性肿瘤组与良性肿瘤组及正常卵巢组织间比较差异显著 (P<0 .0 5 ) . p16蛋白表达随病变发展表达率升高 ,正常卵巢组织组 10 .0 % (1/10 ) ,良性肿瘤组 17.4% (4/2 3) ,交界性肿瘤组 45 .0 % (9/2 0 )及恶性肿瘤组 6 5 .5 % (5 5 /84) ,恶性肿瘤组与良性肿瘤组及正常卵巢组织组间比较有显著差异 (P<0 .0 1) .Cyclin D1 与 p16在卵巢上皮性肿瘤中的表达具有显著正相关关系 (P<0 .0 1,r=0 .5 0 0 31) .结论 Cyclin D1 和 p16在卵巢上皮性肿瘤癌变过程中 ,两者表达水平的相关性反映了癌基因与抑癌基因相互拮抗、抑制的机制
Objective To investigate the expression and correlation of Cyclin D1 and p16 in epithelial ovarian carcinogenesis.Methods Immunohistochemical SABC method was used to detect the expression of Cy-clin D1, p16 in borderline, benign ovarian tumors and normal ovarian tissues (49/84, 53.3%). With the progression of the disease (normal ovarian tissue-benign tumor-borderline tumor-epithelial carcinoma), the expression of Cyclin D1 showed an upward trend ( Respectively, 10 .0%; 26.1%; 45.0%; 5 8.3%). There was significant difference between malignant tumor group and benign tumor group and normal ovarian tissue (P <0.05) .The expression of p16 protein With the development of lesion, the expression rate was increased in 10.0% (1/10) of normal ovarian tissue group, 17.4% (4/2 3) in benign tumor group, 45.0% (9/2 0) in borderline tumor group and malignant There was a significant difference between the malignant tumor group and the benign tumor group and the normal ovarian tissue group (P <0.01) .5 In the tumor group, 65.5% (55/84) showed significant difference (P <0.01) .Cyclin D1 and p16 in ovarian epithelial tumor (P <0.01, r = 0.50031) .Conclusion Cyclin D1 and p16 expression in ovarian epithelial In the process of carcinogenesis of cancers, the correlation between the expression level of the two genes reflects the mechanism of mutual antagonism and inhibition of oncogenes and tumor suppressor genes