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目的制定慢支胶囊中柚皮苷的含量测量方法。方法采用高效液相色谱(HPLC)法测定慢支胶囊中柚皮苷的含量,色谱条件为:C18色谱柱(4.6 mm×150 mm),流动相为甲醇-水(40∶60),检测波长为283 nm。并对准确度、精密度、专属性等进行方法学考察。结果柚皮苷在0.1952~1.9520μg范围内与峰面积呈良好的线性关系,回归方程为Y=6.466×106X+8.342×103,r=0.9999,平均回收率为98.38%,柚皮苷含量为15.05 mg/g。结论该方法灵敏、准确、重现性好、精密度高、专属性强,可作为慢支胶囊中柚皮苷的定量测定方法。
Objective To develop a method for the determination of naringin in chronic bronchitis capsule. Methods HPLC was used to determine the content of naringin in Chuanzhai Capsules. The chromatographic conditions were as follows: C18 column (4.6 mm × 150 mm), the mobile phase consisted of methanol-water (40:60), detection wavelength 283 nm. And the accuracy, precision, specificity and other methodological study. Results The naringin showed a good linear relationship with the peak area in the range of 0.1952 ~ 1.9520μg. The regression equation was Y = 6.466 × 106X + 8.342 × 103, r = 0.9999 with the average recovery of 98.38% and naringin content of 15.05 mg / g. Conclusion The method is sensitive, accurate, reproducible, accurate and specific. It can be used as a quantitative method for the determination of naringin in chronic bronchitis capsules.