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应用籼粳交 IR6 4 / Azucena的 DH群体及其构建的分子标记遗传图谱 ,在遮光条件下 ,通过适温和低温逆境下发芽 ,测定中胚轴长度。采用 QTL Mapper基因定位软件检测控制中胚轴长度的加性效应 QTL s和加性×加性上位性 QTL s,在第 1、3、6、7、8、12等 6条染色体上定位了 8个控制中胚轴长度的 QTL s,其中在第 1、3、7、8染色体上定位了 4个具有加性效应的 QTL s,位于第 7染色体的 1个加性效应 QTL的增长等位基因来自于父本 Azucena,它能使中胚轴伸长 0 .2 6 cm,其贡献率达 17.5 % ,其余 3个加性效应 QTL s的增长等位基因来自于母本 IR6 4 ,能使中胚轴伸长 0 .10～ 0 .2 1cm,在第 3、7、12等3条染色体中共检测到 2对加性×加性上位性效应 ,其贡献率分别为 2 1.6 2 %和 2 .2 7% ,同时各检测到 2对加性效应×环境的互作效应和上位性与环境的互作效应。对应用分子标记辅助育种选育中胚轴伸长的矮秆水稻的可能性进行了讨论
The DH population of indica / japonica hybrids IR6 4 / Azucena and its constructed molecular marker genetic map were used to determine the mesocotyl length under photosynthetic conditions through germination under moderate and low temperature stress. QTL Mapper gene mapping software was used to detect QTLs for additive effects and QTLs for additive epistatic QTLs controlling the length of hypocotyls. The QTLs were mapped on 6 chromosomes 1, 3, 6, 7, 8, 12 QTLs controlling the mesocotyl length, in which QTLs with additive effects were mapped on chromosomes 1, 3, 7 and 8, and the one allele of QTL with additive effects on chromosome 7 From the male parent Azucena, it can make the mesocotyl elongation 0.26 cm, its contribution rate of 17.5%, the other three additive effect QTL s growth allele from the female parent IR6 4, can make Hypocotyl elongation ranged from 0.1 to 0.2 cm, and 2 pairs of additive × additive epistatic effects were detected on the 3, 7, 12 and 3 chromosomes. The contribution rates were 2 1.6 2% and 2, respectively. 2 7%. At the same time, each detected two pairs of additive effects × environment interaction and epistasis and environment interaction effect. The possibility of using molecular marker-assisted breeding to breed hypocotyls of dwarf rice has been discussed