论文部分内容阅读
利用Operon系列引物筛选到1个与HB红花性状基因连锁的RAPD标记OPA15-1160,对差异条带进行克隆与核苷酸测序,根据测序结果设计SCAR引物,在HB红花近等基因系及其白花轮回亲本中进行PCR扩增程序优化和鉴定,筛选出一对引物可稳定扩增出与HB红花性状基因连锁的特异片段,获得了与HB红花性状基因紧密连锁的SCAR标记HB-330。利用具黄色花瓣紫红色基斑的海岛棉与粉红花瓣的红叶棉等种质材料以及HB红花近等基因系与白花轮回亲本杂交的F1、BC1F1、F2群体,对该SCAR标记的特异性与准确性进行了鉴定与验证,在红花植株中扩增出了330bp大小的片段而在白花植株中未扩增出,证明该标记准确性高、重复性好。HB红花是通过远缘杂交转自野生二倍体比克氏棉的性状,已成功地应用于性状标记杂交棉育种。该SCAR标记不仅为HB红花标记杂交种的纯度鉴定提供了有效技术手段,也为新品种保护提供了技术支持,促进了红花性状杂交种的分子标记辅助育种进程。
One RAPD marker, OPA15-1160, linked to the red safflower trait gene was screened by Operon series primers. The differential bands were cloned and sequenced. According to the sequencing results, SCAR primers were designed. The white flower reincarnation parental PCR amplification program optimization and identification, screening a pair of primers can be stably amplified with the HB safflower trait gene linked to specific fragments, obtained with the Saffron trait gene tightly linked SCAR marker HB- 330. The F1 and BC1F1 and F2 populations of F1, BC1F1 and F2 crossed with the germplasm material of sea-island cotton with pink petals of red petals and red leaf cotton of pink petals, The accuracy was verified and verified. A 330bp fragment was amplified in safflower plants and not amplified in white flower plants, which proved that the marker was highly accurate and reproducible. HB safflower was transferred from wild diploid Beech to cotton by distant hybridization and has been successfully applied to hybrid cotton breeding with trait marker. The SCAR marker not only provides an effective technical means for the purity identification of HB safflower hybrids, but also provides technical support for the protection of new varieties and promotes the molecular marker-assisted breeding process of safflower hybrids.